均匀试验优化四味穿心莲散半仿生提取工艺研究  

Study on semi-bionic extraction technology of SiweiChuanxinlian San optimized by uniform experimental design

作　　者：迟进坤 陈涛[2] 博意 刘广旭 单文琪 王宏军 CHI Jinkun;CHEN Tao;BO Yi;LIU Guangxu;SHAN Wenqi;WANG Hongjun(College of Animal Husband&Veterinary Medicine,Jinzhou Medical University,Jinzhou,Liaoning Province 121001,China;Hospital of the Fifth Division of Xinjiang Production and Construction Corps,Bole,Xinjiang 833400,China;Wafangdian Center for Animal Disease Control and Prevention,Wafangdian,Liaoning Province 116300,China;Dalian Sanyi Animal Medicine Co.,LTD.,Dalian,Liaoning Province 116036,China)

机构地区：[1]锦州医科大学畜牧兽医学院,辽宁锦州121001 [2]新疆生产建设兵团第五师医院,新疆博乐833400 [3]瓦房店市动物疫病预防控制中心,辽宁瓦房店116300 [4]大连三仪动物药品有限公司,辽宁大连116036

出　　处：《中国饲料》2025年第7期78-82,共5页China Feed

基　　金：辽宁省教育厅科研项目(LJ212410160081,LJ212410160090);辽宁省科技厅博士启动项目(2023-BSBA-160);辽宁省种质创新藏梁于技专项计划项目(2023JH1/10200006)。

摘　　要：为优化四味穿心莲散半仿生提取物(SBSCS)的制备工艺,本试验利用DPS19.05版软件进行3因素5水平的均匀试验设计测定各组浸膏率,以微孔-平板法测定SBSCS体外对猪大肠杆菌的最小抑菌浓度(MIC),高效液相色谱法(HPLC)检测SBSCS中绿原酸、芦丁含量,流动相梯度洗脱液为乙腈-0.1%磷酸水;以浸膏率、MIC、绿原酸及芦丁含量组成的综合评分为响应值,以提取溶媒pH、料液比、提取时间为因变量,优化分析SBSCS制备工艺参数。结果显示:SBSCS制备最优的工艺为pH 6.8、料液比1:20、提取时间2.0 h。此条件下,浸膏率为(37.81±0.12)%、MIC为12.5 mg/mL、绿原酸含量为(85.22±0.23)μg/mL、芦丁含量为(3.12±0.26)μg/mL。该项研究获得了制备SBSCS的最优工艺,为其作用机制及新产品研发奠定了基础。The purpose of this study was to obtain the preparation technology of semi-biomimetic extract on Siwei Chuanxinlian San(SBSCS).The extractum yield of each group was determined by the uniform test design of 3 factors and 5 levels using DPS19.05 software.The minimum inhibitory concentration(MIC)of the each group extract against porcine E.coli were determined by microporous plate method in vitro.Acetonitrile-0.1%phosphoric acid water as mobile phase gradient elution,and the contents of chlorogenic acid and rutin in the each group extract were detected by high performance liquid chromatography(HPLC).The comprehensive scores composed of extract rate,MIC,content of chlorogenic acid and rutin were used as response values.And the extraction process parameters were optimized with solvent pH,solid-liquid ratio and extraction time as dependent variables.The results showed that the optimal preparation process of SBSCS was pH 6.8,solid-liquid ratio 1:20,extraction time 2.0 h.Under this experimental conditions,the extract yield was(37.81±0.12)%,MIC against porcine E.coli was 12.5 mg/mL,chlorogenic acid content was(85.22±0.23)μg/mL,rutin content was(3.12±0.26)μg/mL.This study obtained the optimal SBSCS,which laid a foundation for its mechanism of action and the development of new products.

关 键 词：四味穿心莲散 半仿生提取 均匀试验设计 大肠杆菌 

分 类 号：S816.7[农业科学—饲料科学]