不同光强梯度对番茄两段式嫁接苗愈合及生长的影响  

Effects of different light intensities on the healing and growth of grafted seedlings in two-stage grafting of tomato seedlings

作　　者：吴帼秀 张家梓 李严曼[1,2] 李阳 崔丹丹 何雪颖 王凡 李胜利 WU Guoxiu;ZHANG Jiazi;LI Yanman;LI Yang;CUI Dandan;HE Xueying;WANG Fan;LI Shengli(College of Horticulture,Henan Agricultural University,Zhengzhou 450046,China;Henan Research Center of Protected Horticulture Engineering Technology,Zhengzhou 450046,China)

机构地区：[1]河南农业大学园艺学院,郑州450046 [2]河南省设施园艺工程技术研究中心,郑州450046

出　　处：《农业工程学报》2025年第4期230-238,共9页Transactions of the Chinese Society of Agricultural Engineering

基　　金：河南省大宗蔬菜产业技术体系项目(HARS-22-07-S);河南省中央引导地方科技发展资金项目(Z20231811003);河南省科技攻关项目(242102111093)。

摘　　要：为解决番茄两段式嫁接育苗中如何在高密度环境下快速愈合及扦插后快速生根的问题,该研究以番茄品种‘粉都153’为接穗、‘金钻’为砧木,对其进行双断根嫁接,研究嫁接苗在愈合期(1~2、3~4、5 d)不同光强梯度30-60-90(CK)、40-80-120(T1)、50-100-150(T2)、60-120-180(T3)、70-140-210μmol/(m^(2)·s)(T4)下的愈合情况,并将不同光强下的嫁接苗分别高密度愈合1、3和5 d后扦插,判断植株生长情况。结果表明,T1、T2处理的嫁接苗成活率较高,且与CK差异不显著(P>0.05);T1处理下嫁接苗的愈合最快,且切口处具有较高的抗氧化酶活性及光合色素含量,较低的超氧阴离子含量;不同光强处理3 d后扦插嫁接苗的成活率较高,其中T1和T2处理的最高,不同光强处理1 d后扦插各处理间无显著差异(P>0.05),处理5 d后扦插成活率降低;对扦插嫁接苗的生长进行综合评价,T1处理1、3、5 d后扦插,嫁接苗的根系及地上部生长均最好,其次为T2、T3在第5天时扦插也表现出较好的效果。综合考虑,在番茄两段式嫁接的高密度愈合阶段,在1~2、3~4、5 d愈合期采用40-80-120μmol/(m^(2)·s)的光强管理模式对愈合最为有利,且最好在3 d内进行扦插,可更好地保证成活率及嫁接苗的质量。Tomato is one of the most popular vegetable crops with the largest facility cultivation in China.Succession barriers,soil-borne diseases,and environmental stresses can often occur in actual production,leading to decreasing quality and yield.Fortunately,grafting has been one of the most effective operations to control the major diseases for the high resistance in recent years.Among them,two-stage grafting of tomatoes can be expected to cultivate high-quality seedling resistance.It is of great significance to precise control the light environment for better grafting during the healing and cutting stages.However,it is unclear on the specific parameters of the light environment during two-stage grafting.This study aims to explore the effects of different light intensities on the healing and growth of grafted seedlings in the two-stage grafting of tomatoes.The double-cut grafted seedlings rapidly healed and then took root after cutting under a high-density environment.The strong seedlings were cultivated after optimization.The grafted seedlings were then placed in a self-developed container made of acrylic and highdensity foam board.The light intensity was selected to regulate the light environment at the ratio of red,blue,and green light during the healing period.The tomato variety'Fendu 153'was used as the scion,while the'Jinzuan'was used as the rootstock.The healing status and root regeneration of grafted seedlings were investigated under different light intensity gradients of 30-60-90(CK),40-80-120(T1),50-100-150(T2),60-120-180(T3),and 70-140-210(T4)μmol/(m^(2)·s)during the healing period(1-2,3-4,and 5 d).The grafted seedlings were cut under different light intensities after high-density healing for 1,3,and 5 d,respectively,in order to evaluate the growth of plants.The growth indexes were measured,such as the plant height,stem diameter,leaf area,shoot ratio,and length,as well as surface area,diameter,and volume of root.The results showed that a higher survival rate of grafted seedlings was achieved in the T1 and

关 键 词：番茄 光强 两段式嫁接 愈合 生长 

分 类 号：S641.2[农业科学—蔬菜学]