鄂尔多斯细毛羊前后盘吸虫病病原分子鉴定及驱虫药物筛选  

Molecular Identification of the Pathogen of Paramphistomiasis in Ordos Fine-wool Sheep and Screening of Deworming Drugs

作　　者：乌日力格 李娜[1] 格日勒格拉巴 哈斯图雅 张靖靖 侯斌 哈斯苏荣[1] WURI Lige;LI Na;GERILE Gelaba;HASI Tuya;ZHANG Jingjing;HOU Bin;HASI Surong(National Animal Medicine Experimental Teaching Center,Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Diseases,Ministry of Agriculture and Rural Affairs,College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Wushen Animal Disease Prevention and Control Center,Ordos 017300,China;Ordos Vocational College of Eco-Environment,Ordos 017010,China)

机构地区：[1]内蒙古农业大学兽医学院,农业农村部动物疾病临床诊疗技术重点实验室,国家级动物医学实验教学中心,呼和浩特010018 [2]乌审旗动物疫病预防控制中心,鄂尔多斯017300 [3]鄂尔多斯生态环境职业学院,鄂尔多斯017010

出　　处：《中国畜牧兽医》2025年第4期1807-1814,共8页China Animal Husbandry & Veterinary Medicine

基　　金：鄂尔多斯市科技重大专项(2022EEDSKJZDZX026);乌审旗动物疫病预防控制中心横向课题。

摘　　要：【目的】探明鄂尔多斯细毛羊体内寄生的前后盘吸虫种类并筛选出理想的驱虫药物。【方法】在粪便虫卵检查初步诊断前后盘吸虫病的基础上,通过剖检采集鄂尔多斯细毛羊瘤胃内的前后盘吸虫虫体,并进行形态学和分子生物学鉴定。随机选取3条虫体提取DNA,继而对核糖体内转录间隔区2(ITS-2)进行PCR扩增,将扩增产物测序后于NCBI数据库进行BLAST比对分析,并构建系统进化树。分别用氯硝柳胺、硝氯酚、碘醚柳胺和3%敌百虫联合硝氯酚,针对感染前后盘吸虫病的绵羊开展驱虫效果比较试验。【结果】ITS-2基因PCR扩增获得大小为441 bp的条带,与目的基因条带相符。3条虫体与源于中国的鹿前后盘吸虫ITS-2(KJ459936.1)序列相似性依次为99.3%、99.3%和99.8%。进化分析结果表明,该前后盘吸虫与来自中国(KP341671)、爱尔兰(AB973398)、阿根廷(HM209066)、德国(MZ532797)的前后盘吸虫同属一个大分支,且与鹿前后盘吸虫(Paramphistomum cervi)共处一个小分支内,鉴定为鹿前后盘吸虫。驱虫试验中氯硝柳胺、硝氯酚、碘醚柳胺、3%敌百虫配伍硝氯酚组的虫卵减少率分别为44.00%、26.73%、23.00%和90.80%。【结论】在内蒙古乌审旗地区鄂尔多斯细毛羊体内寄生的前后盘吸虫属鹿前后盘吸虫,采用3%敌百虫联合硝氯酚治疗可达到较好的驱虫效果。【Objective】The purpose of this study was to explore the species of Paramphistomum in Ordos fine-wool sheep and screen out ideal anthelmintic drugs.【Method】Based on the preliminary diagnosis of paramphistomiasis by fecal egg examination,the Paramphistomum in rumen of Ordos fine-wool sheep were collected through necropsy for morphological and molecular biological identification.Three parasites were randomly selected to extract DNA,and then the internal transcribed spacer 2(ITS-2)of ribosome was amplified by PCR.After sequencing the amplified products,BLAST alignment analysis was performed in NCBI database,and the phylogenetic tree was constructed.Niclosamide,nitroclofene,rafoxanide and 3%trichlorfon combined with nitroclofene were respectively used to conduct a comparative test on the anthelmintic effects in sheep infected with paramphistomiasis.【Result】The band size of 441 bp was obtained by PCR amplification of ITS-2 gene,which was consistent with the target gene band.The sequence similarity between the three parasites and the ITS-2(KJ459936.1)from China was 99.3%,99.3%and 99.8%,respectively.The results of phylogenetic analysis showed that this Paramphistomum was in the same large branch as Paramphistomum from China(KP341671),Ireland(AB973398),Argentina(HM209066)and Germany(MZ532797),and was in a small branch with Paramphistomum cervi,indicating that the Paramphistomum obtained in this study was Paramphistomum cervi.In the anthelmintic test,the egg reduction rates in niclosamide,nitroclofene,rafoxanide and 3%trichlorfon combined with nitroclofene groups were 44.00%,26.73%,23.00%and 90.80%,respectively.【Conclusion】The Paramphistomum parasitizing in Ordos fine-wool sheep in Wushen Banner,Inner Mongolia was Paramphistomum cervi,and the treatment with a combination of 3%trichlorfon and rafoxanide could achieve a better anthelmintic effect.

关 键 词：鄂尔多斯细毛羊 前后盘吸虫 分子生物学鉴定 驱虫效果 

分 类 号：S852.735[农业科学—基础兽医学] S855.9[农业科学—兽医学]