气管上皮细胞的分离培养及其在呼吸道冠状病毒感染中作用的研究进展  

Research Progress on Isolation and Culture of Tracheal Epithelial Cells and Their Role in Respiratory Coronaviruses Infection

作　　者：郭倩倩 汤雨晴[1,2] 齐振昌 李庆豪 金鑫 马俊兴 孙娟[1,2] GUO Qianqian;TANG Yuqing;QI Zhenchang;LI Qinghao;JIN Xin;MA Junxing;SUN Juan(College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,China;Ministry of Education Key Laboratory for Animal Pathogens and Biosafety,Zhengzhou 450046,China;Fuwai Central China Cardiovascular Hospital,Zhengzhou 451464,China)

机构地区：[1]河南农业大学动物医学院,郑州450046 [2]动物病原与生物安全教育部重点实验室,郑州450046 [3]阜外华中心血管病医院,郑州451464

出　　处：《中国畜牧兽医》2025年第4期1836-1843,共8页China Animal Husbandry & Veterinary Medicine

基　　金：河南省科技攻关项目(242102111008);河南农业大学博士启动金(30500750)。

摘　　要：近年来呼吸道冠状病毒成为研究热点,其主要感染宿主气管的纤毛细胞和杯状细胞,使细胞间丧失紧密连接,损害呼吸系统屏障的完整性,进而引发下呼吸道感染。气管上皮细胞(trachea epithelium cells, TECs)作为机体内阻挡病原入侵的重要屏障,同时也是许多病原体的靶细胞,是研究呼吸道冠状病毒的重要模型。在宿主与病毒的相互作用中,呼吸道冠状病毒优先攻击纤毛细胞,导致纤毛清除功能障碍,破坏气管上皮的防御屏障,从而加剧继发性感染。笔者概述气管上皮细胞的形态结构和生理功能,重点总结了气管上皮细胞体外分离培养的方法及其优缺点,并进一步探讨了呼吸道病原体与宿主之间的作用机制,以期为气管上皮细胞的分离、培养提供参考,同时为研究呼吸道冠状病毒的感染机制和防治策略提供支撑。In recent years,respiratory Coronaviruses have become a focus of research.They mainly infect ciliated cells and goblet cells of the host trachea,resulting in loss of tight connections between cells,damage the integrity of the respiratory system barrier,and then cause low respiratory tract infection.The trachea epithelium cells(TECs),as an important barrier against pathogen invasion in the body,are also the target cells of many pathogens,and are an important model for the study of respiratory Coronaviruses.During the host-virus interactions,respiratory Coronaviruses preferentially attack ciliary cells,resulting in ciliary clearance dysfunction and destruction of the defensive barrier of the tracheal epithelium,thus exacerbating secondary infection.The authors summarized the morphological structure and physiological function of tracheal epithelial cells,and focused on the methods of isolation and culture of tracheal epithelial cells in vitro,as well as their advantages and disadvantages.The mechanism of interaction between respiratory pathogens and their hosts was further discussed,in order to provide a reference for the isolation and culture of tracheal epithelial cells,and provide support for the study of the infection mechanism and prevention strategy of respiratory Coronavirus.

关 键 词：气管上皮细胞 分离 培养 呼吸道冠状病毒 

分 类 号：S852.659.6[农业科学—基础兽医学]