Effect of Proline Pretreatment on the Water Stress Response in “Siete Caldos” Pepper Plants  

作　　者：Blanca Olivia Trejo-Paniagua Nancy Ruiz-Lau María Goretty Caamal-Chan Rosa Isela Cruz-Rodríguez Anayancy Lam-Gutiérrez Víctor Manuel Ruíz-Valdiviezo 

机构地区：[1]Tecnológico Nacional de México Campus Tuxtla Gutiérrez,Carretera Panamericana Km 1080,Tuxtla Gutiérrez,29050,Chiapas,México [2]SECIHTI-Tecnológico Nacional de México Campus Tuxtla,Carretera Panamericana Km 1080,Tuxtla Gutiérrez,29050,Chiapas,México [3]SECIHTI-Centro de Investigaciones Biológicas del Noroeste S.C.,Instituto Politécnico Nacional 195,Playa Palo de Santa Rita Sur,La Paz,23096,Baja California Sur,México [4]Tecnológico Nacional de México-Instituto Tecnológico Superior de Cintalapa,Cintalapa de Figueroa,30400,Chiapas,México

出　　处：《Phyton-International Journal of Experimental Botany》2025年第3期861-873,共13页国际实验植物学杂志(英文)

摘　　要：Exogenous proline is an effective agent for increasing plant tolerance to abiotic stress in plants. In this study, we evaluated its effect on seedlings of Siete Caldos chili pepper (Capsicum frutescens), a semi-domesticated variety. The Capsicum genus is known for its sensitivity to water stress. We pretreated the seedlings’ roots by immersing them in proline solutions (0, 2.5, 5, 7.5, and 10 mM) for 48 h. Then, we exposed them to water stress using a Hoagland nutrient solution supplemented with 10% polyethylene glycol (PEG-8000) for nine days. We analyzed key physiological and biochemical parameters, including relative water content, cell membrane stability index, electrolyte leakage, chlorophyll, and proline content. The results indicated that proline concentrations of 2.5 and 5 mM significantly increased tolerance to water stress, with 100% survival. These seedlings maintained greater hydration and cell membrane stability compared to non-pretreated seedlings. In contrast, at the highest concentrations (7.5 and 10 mM Pro), survival was 63.63% and 54.54%, respectively. This study demonstrated that exogenous proline enhances water stress tolerance in Capsicum frutescens seedlings by mitigating the negative impact on physiological and biochemical processes vital for survival. This theoretical foundation can be applied to improve chili seedling performance in controlled production environments.

关 键 词：Capsicum frutescens exogenous proline TOLERANCE siete caldos chili pepper 

分 类 号：S64[农业科学—蔬菜学]