Research on the Cymbidium tortisepalum var.longibracteatum Growth and Non-Tube Rapid Propagation Based on Response Surface Methodology  

作　　者：Guolan Wang Ting Xie Lijun Fu Siying Qu Jie Li 

机构地区：[1]College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,621000,China

出　　处：《Phyton-International Journal of Experimental Botany》2025年第3期953-971,共19页国际实验植物学杂志(英文)

基　　金：funded by the Sichuan Science and Technology Program“Research on Synergistic Relationship and Reintroduction Technology of C.tortisepalum var.longibracteatum and Endophytic Mycorrhizal Fungi in Eastern Sichuan”(2017JY0132);Longshan Academic Talent Research Supporting Program of SWUST(17LZX521,18LZX522).

摘　　要：The objective of this study was to determine the optimal proportions of plant growth regulators for growth and non-tube rapid propagation of Cymbidium tortisepalum var. longibracteatum;seedlings were utilized as the material. The effects of various combinations and concentrations of 6-benzylaminopurine (6-BA), gibberellic acid (GA_(3)), and naphthaleneacetic acid (NAA) on growth and non-tube rapid propagation were assessed through a single-factor testing and response surface methodology. The results indicated that 6-BA at 60 mg/L, GA_(3) at 150 mg/L, and NAA at 30 mg/L were the most effective concentrations for promoting leaf buds formation in the single-factor analysis. Response surface methodology clarified the sensitivity of the proliferation rate of lateral buds to the three factors, with 6-BA being the most influential, followed by GA_(3) and NAA. The increase in leaf area was most significantly influenced by NAA, then GA_(3), and least by 6-BA, while the increase in plant height was most responsive to GA_(3), followed by 6-BA, and then NAA. The ideal concentrations of plant growth regulators were established as 6-BA at 43 mg/L, GA_(3) at 169 mg/L, and NAA at 36 mg/L. Under these conditions, the lateral bud number per plant was 2.78, with a leaf area increment of 2.87 cm2 and a plant height increment of 2.67 cm.

关 键 词：Cymbidium tortisepalum var.longibracteatum GROWTH non-tube rapid propagation response surface methodology plant growth regulator 

分 类 号：S68[农业科学—观赏园艺]