不同中药方剂对鸡人工感染大肠杆菌病药效试验  

Pharmacodynamic experiment of different traditional Chinese medicine prescriptions in preventing and treating chicken artificial infection of Escherichia coli

作　　者：赵增成[1] 杨世发[1] 殷斌[1] 刘月月[1] 黄中利[1] 宋士凯 闫遵祥 张荣岭 王海挺 衣云鹏 林树乾[1] ZHAO Zengcheng;YANG Shifa;YIN Bin;LIU Yueyue;HUANG Zhongli;SONG Shikai;YAN Zunxiang;ZHANG Rongling;WANG Haiting;YI Yunpeng;LIN Shuqian(Poultry Institute,Shandong Academy of Agricultural Sciences,Jinan Shandong 250010,China)

机构地区：[1]山东省农业科学院家禽研究所,山东济南250010

出　　处：《中兽医医药杂志》2025年第2期78-84,共7页Journal of Traditional Chinese Veterinary Medicine

基　　金：山东省乡村振兴科技创新提振行动计划(2023TZXD083);山东省科技型中小企业创新能力提升项目(2023TSGC0092)。

摘　　要：为了筛选出防治鸡大肠杆菌病的有效中药方剂,采用口腔灌服大肠埃希菌CVCC1558株,建立SPF雏鸡大肠杆菌人工发病模型,选用升降散、甘露消毒丹、银翘散、达原饮4种方剂为试验药物,进行临床药效试验。试验共设6个组,其中A组仅灌服升降散,B组灌服升降散和甘露消毒丹,C组灌服升降散和银翘散,D组灌服升降散和达原饮,E组为阳性对照组,F组为阴性对照组,各组鸡日常饲养管理情况相同。对试验鸡的精神状态、粪便状况、体温、增重、血液白细胞计数、病理剖检变化进行观测,试验期共9d。结果显示,除阴性对照组雏鸡精神正常外,各攻毒组试验鸡均表现出精神沉郁、腹泻等症状,无病鸡死亡。A组、B组、C组、D组精神正常鸡平均占比分别为71.1%、80.0%、76.7%、83.3%,阳性对照组精神正常鸡占比仅为55.6%。各药物组鸡腹泻程度显著轻于阳性对照组(P<0.05),各药物组雏鸡腹泻程度计分分别为2.33±0.71、2.33±0.50、2.33±0.50、2.44±0.53,阳性对照组雏鸡腹泻程度计分为3.00。各药物组雏鸡平均体温分别为(41.90±0.30)℃、(41.72±0.25)℃、(41.89±0.30)℃、(41.95±0.30)℃,阳性对照组和阴性对照组雏鸡平均体温分别为(42.09±0.27)℃、(41.84±0.25)℃,表明各药物组均有清热效果。攻毒后第6天,各药物组雏鸡平均增重率分别为35.00%、35.27%、35.56%、40.48%,阳性对照组和阴性对照组雏鸡平均增重率分别为35.97%、40.66%;攻毒后第9天,各药物组雏鸡平均增重率分别为45.71%、47.41%、46.59%、52.64%,阳性对照组和阴性对照组雏鸡平均增重率分别为47.89%、51.33%。血液学检查结果显示,各药物组雏鸡白细胞计数分别为78.30×10^(9)/L、69.10×10^(9)/L、71.00×10^(9)/L、74.30×10^(9)/L,阳性对照组和阴性对照组雏鸡白细胞计数分别为87.60×10^(9)/L、57.00×10^(9)/L,各药物组雏鸡白细胞数低于阳性对照组,但高于阴性对照组,表明各药物组炎In order to screen out the effective traditional Chinese medicine prescriptions for the prevention and treatment of chicken colibacillosis,oral gavage of Escherichia coli CVCC1558 strain was used to carry out the artificial pathogenesis of SPF chicken colibacillosis,and four kinds of formulas were selected as the experimental drugs,including Shengjiang San,Ganlu Xiaodu Dan,Yinqiao San and Dayuan Yin,for the clinical efficacy test.Six groups were set up in the experiment,in which group A was only given Shengjiang San,group B was given Shengjiang San and Ganlu Xiaodu Dan,group C was given Shengjiang San and Yinqiao San,group D was given Shengjiang San and Dayuan Yin,group E was the positive control group,and group F was the negative control group.The same feeding and management were conducted in all groups,and the experiment lasted for 9 consecutive days.The mental performance,fecal status,body temperature,weight gain,white blood cell count,pathological and anatomical changes of the experimental chickens were observed and determined.The results showed that all experimental chickens showed symptoms of depression and diarrhea after challenge,no sick chickens died,and the chicks in the negative control group were in normal spirit.In group A,B,C and D,the average proportion of mentally normal chickens was 71.1%,80.0%,76.7%and 83.3%,respectively,and the positive control group was 55.6%.The degree of diarrhea in each medication group was significantly lighter than that in the positive control group(P<0.05),and the scores of diarrhea degree in each medication group were 2.33±0.71,2.33±0.50,2.33±0.50,2.44±0.53,and 3.00 in the positive control.The average body temperature of each medication group was(41.90±0.30)℃,(41.72±0.25)℃,(41.89±0.30)℃and(41.95±0.30)℃,while it was(42.09±0.27)℃in the positive control group,and(41.84±0.25)℃in the negative control group,respectively,indicating that all drugs had heat clearing effect.On the sixth day after challenge,the weight gain rates of the medication groups were

关 键 词：鸡大肠杆菌病 升降散 达原饮 药效试验 

分 类 号：S853.7[农业科学—临床兽医学]