Advances in the development of infectious clones of human coronaviruses and related applications  

作　　者：Guanya Liu Ruixiao Tan Yiyi Wu Mengwei Wang Baoying Huang Wenjie Tan 

机构地区：[1]School of Public Health,Baotou Medical College,Baotou 011400,China [2]National Key Labratory of Inteigent Tracking and Forasting for Inftious Dseases,National Health Commsions Key Labraty of Biosafety,Natonal Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Bejing 102206,China [3]College of Life Sciences,Bejing Normal University,Beijing 100875,China

出　　处：《Biosafety and Health》2025年第1期59-73,共15页生物安全与健康(英文)

基　　金：the National Key Research and Development Program of China(2022YFC2304101,2021YFA1201003).

摘　　要：Coronaviruses can infect humans,mammals,and birds,leading to respiratory,gastrointestinal,and neurological diseases.These viruses are significant zoonotic pathogens with nine known types capable of infecting humans.The coronavirus genome,approximately 30 kb in size,is the largest known ribonucleic acid(RNA)virus genome,and its complexity makes assembly and manipulation time-consuming and labor-intensive.Reverse genetic systems are widely used to engineer recombinant viruses that can be adapted at Biosafety Level 2(BSL-2)for studying viral gene function,replication,pathogenesis,vaccines,and therapeutics.The infectious clones,which enabled the recovery of various viruses after DNA recombinant technology,were indispensable tools for the reverse genetics of viruses.Various techniques for constructing infectious clones of human coronaviruses(HCoV)have been developed,encompassing methods such as vaccinia virus vectors method,in vitro ligation,bacterial artificial chromosome systems,yeast artificial chromosome systems,circular polymerase extension reaction,and the recently reported infectious sub-genomic amplicons technology.This review summarizes the status of various techniques for constructing infectious clones of human coronaviruses and related applications.

关 键 词：Human coronaviruses Reverse genetics Infectious clones APPLICATION 

分 类 号：R373[医药卫生—病原生物学]