玉米穗部叶片相关性状的GWAS分析  

Genome Wide Association Analysis of Ear Leaf Traits in Maize

作　　者：李昊[1] 刘瑞来 许家伟 张振弘 李春翔 张林[1] LI Hao;LIU Rui-lai;XU Jia-wei;ZHANG Zhen-hong;LI Chun-xiang;ZHANG Lin(Northeast Agricultural University/Key Laboratory of Germplasm Innovation and Physiological Ecology of Food Crops in Cold Region,Ministry of Education/Engineering Technology Research Center of Maize Germplasm Resources Innovation on Cold Land of Heilongjiang Province,Harbin 150030,China)

机构地区：[1]东北农业大学/寒地粮食作物种质创新与生理生态教育部重点实验室/黑龙江省寒地玉米种质资源创新工程技术研究中心,哈尔滨150030

出　　处：《玉米科学》2025年第3期26-32,共7页Journal of Maize Sciences

基　　金：国家重点研发计划项目“东华北春玉米区早熟高产抗逆宜机收新种质创制与应用”(2021YFD1201000)。

摘　　要：以294份自交系构成的玉米自然群体为试材,调查穗部3片叶片的叶长、叶宽和叶面积等表型性状,结合5 850 982个SNPs标记的基因型数据进行全基因组关联分析(GWAS)。结果表明,参试材料的穗部叶片性状存在显著差异,表现出典型的数量性状特点,性状间表现为显著相关(P<0.05)。检测出极显著相关的SNP位点439个(P<1E-05),基于12个与多个性状均显著相关的SNP位点预测到29个候选基因,其中18个具有功能注释,筛选出5个重要的候选基因,包括MYB家族转录因子、NAC家族转录因子、GLK家族转录因子、E3泛素蛋白连接酶、钙调蛋白结合核蛋白等,可能通过相关信号转导、物质代谢合成及调控相关蛋白质稳定性来调节玉米穗部叶片性状。Two hundred and ninety-four inbred lines were used to investigate phenotypic traits such as leaf length,leaf width and leaf area of three ear leaves.Then Genome wide association analysis(GWAS)was performed with genotypic data of 5850982 SNPs.The results showed that there were significant differences in panicle leaf traits among the inbred lines,showing typical quantitative traits,and significant correlations among the traits(P<0.05).A total of 439 SNP loci were detected with highly significant correlations(P<1E-05).Based on 12 SNP loci significantly associated with multiple traits,29 candidate genes were predicted,18 of which have functional annota⁃tions.Five important candidate genes were screened,including MYB family transcription factor,NAC family tran⁃scription factor,GLK family transcription factor,E3 ubiquitin ligase and calmodulin binding nuclear protein,which regulate ear leaf traits through related signal transduction,substance metabolism,and regulation of protein stability.

关 键 词：玉米 穗部叶片 关联分析 候选基因 

分 类 号：S513.035.3[农业科学—作物学]