十溴联苯醚(BDE-209)对单环刺螠的急性毒性及消化酶活力的影响  

Acute toxicity of decabromodiphenyl ether(BDE-209)to and its effect on the digestive enzymes activities of Urechis unicinctus

作　　者：许星鸿 刘统昊 丁子媛 刘顺 亓鲁 高弋强 周超仪 XU Xinghong;LIU Tonghao;DING Ziyuan;LIU Shun;QI Lu;GAO Yiqiang;ZHOU Chaoyi(Jiangsu Key Laboratory of Marine Bioresources and Environment,Jiangsu Ocean University,Lianyungang 222005,China;College of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China)

机构地区：[1]江苏海洋大学,江苏省海洋生物资源与环境重点实验室,江苏连云港222005 [2]江苏海洋大学,海洋科学与水产学院,江苏连云港222005

出　　处：《海洋湖沼通报(中英文)》2025年第1期44-52,共9页Transactions of Oceanology and Limnology

基　　金：江苏省科技厅苏北科技专项(LYG-SZ201814);连云港市花果山英才计划(KK20100);江苏省优势学科建设工程资助项目(CXKT2020);连云港市科技局现代农业项目(CN1411);江苏省研究生和大学生科研与实践创新计划项目(KYCX18_2567)。

摘　　要：为充实海洋持久性有机污染物对底栖动物毒性效应的基础资料,本文采用不同质量浓度的十溴联苯醚(BDE-209),检测其对单环刺螠(Urechis unicinctus)的急性毒性效应以及消化酶活力的影响。实验结果表明:BDE-209对单环刺螠的48、72和96 h的LC_(50)分别为153.170、96.791和60.042 mg/L,安全浓度为6.004 mg/L。浓度在60μg/L及以下的BDE-209胁迫对碱性蛋白酶、酸性蛋白酶、纤维素酶和脂肪酶有显著诱导作用,以0.6μg/L组诱导幅度最大,且处理3 d时,胁迫浓度0.6μg/L及以下的实验组碱性蛋白酶活力与剂量浓度之间存在显著正相关关系(P<0.01)。各处理组淀粉酶活力均呈现被抑制效应,且处理1 d时酶活力与剂量浓度之间呈显著负相关关系(P<0.05)。长时间的BDE-209胁迫会造成消化酶活力下降,处理15 d时,除60μg/L及以下的处理组碱性蛋白酶外,其他各处理组消化酶活力均显著低于对照组。虽然6000μg/L BDE-209略低于安全浓度,但对各消化酶活力基本呈现抑制作用。BDE-209对单环刺螠属于低毒性-中毒性物质,一定浓度的BDE-209对除淀粉酶以外的消化酶有诱导作用,但低浓度BDE-209长时间的胁迫以及接近安全浓度的胁迫会抑制消化酶活力。To enrich the basic data of the toxic effects of marine environmental hormones on benthic animals,the acute toxicity of decabromodiphenyl ether(BDE-209)with different mass concentrations to and its effect on the digestive enzyme activities of Urechis unicinctus were analyzed in this study.The experimental results showed that LC50 of BDE-209 for U.unicinctus at 48 h,72 h,and 96 h was 153.170 mg/L,96.791 mg/L,and 60.042 mg/L,respectively,while BDE-209 was safe at the concentration of 6.004 mg/L.BDE-209 at the concentration of 60μg/L or less can induce alkaline protease,acid protease,cellulase and lipase significantly,and the maximum induction degree was found at 0.6μg/L.After 3 days of exposure,there was a positive correlation between alkaline protease activity and dose concentration in the treatment group with stress concentration of 0.6μg/L and below(P<0.01).Amylase activity was inhibited in all treatments,and a significant negative correlation was observed between amylase activity and concentration after 1 day of exposure(P<0.05).The activities of digestive enzymes decreased under the long-time stress of BDE-209.After exposure for 15 d,the activities of digestive enzymes in all treatments were significantly lower than those in control group except alkaline protease in 60μg/L or less treatments.Although 6,000μg/L of BDE-209 was slightly lower than the safe concentration(6.004 mg/L),it showed an inhibited effect on the digestive enzyme activities.BDE209 was low-moderate toxic to U.unicinctus,and a certain concentration of BDE-209 can induce digestive enzymes except amylase,but long-term stress at low concentration or at near-safe concentration can inhibit digestive enzyme activities.

关 键 词：十溴联苯醚 单环刺螠 急性毒性 消化酶活力 

分 类 号：X826[环境科学与工程—环境工程]