海洋酸化和铜离子对三角褐指藻生长和光合活性的影响  

Effects of ocean acidification and Cu 2+on the growth and photosynthetic performance of Phaeodactylum tricornutum

作　　者：赵璐 钟志海 杨剑超[3] 顾成柏[1] ZHAO Lu;ZHONG Zhihai;YANG Jianchao;GU Chengbai(College of Marine Engineering,Rizhao Polytechnic,Rizhao 276826,China;Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;Yantai Academy of Agricultural Sciences,Yantai 265500,China)

机构地区：[1]日照职业技术学院海洋工程学院,山东日照276826 [2]中国科学院烟台海岸带研究所,山东烟台264003 [3]烟台市农业科学研究院,山东烟台265500

出　　处：《海洋湖沼通报(中英文)》2025年第1期88-95,共8页Transactions of Oceanology and Limnology

基　　金：山东省高等学校青年创新团队人才引育计划(S190007170001);烟台市科技创新发展计划(2020MSGY058)。

摘　　要：铜离子和海洋酸化分别对三角褐指藻光合作用的影响已得到了广泛研究,但两者的耦合效应研究较少。本文测定了不同铜离子浓度(0.1,0.25,0.5 mgL^(-1) Cu^(2+))下不同CO_(2)浓度(400和1 500μatm CO_(2))对三角褐指藻生长、光合色素和光合活性的影响。结果表明,在400μatm CO_(2)条件下,0.5 mgL^(-1) Cu^(2+)降低了三角褐指藻的生长速率、叶绿素a和c含量、最大光化学效率(Fv/Fm)、最大电子传递速率(rETR_(max))、单位反应中心捕获的用于电子传递的能量(ETo/RC)和单位反应中心捕获的传递到PSI电子受体侧的能量(REo/RC),但上述参数均显著低于1 500μatm CO_(2)条件下的(p<0.05),说明高浓度Cu^(2+)不仅限制了三角褐指藻的生长,也抑制了光系统II(PSII)的活性,但海洋酸化缓解了上述的抑制作用。在海洋酸化和重金属污染较为严重的近海海域,海洋酸化可以提高三角褐指藻对铜离子的耐受性,使其在复杂多变的环境中得以生存。Effects of Cu^(2+) and ocean acidification on the photosynthesis of Phaeodactylum tricornutum have been widely studied,but the studies on their coupling effects are less.The effects of different CO_(2) concentration(400 and 1500μatm CO_(2))on the specific growth rate(μ),photosynthetic pigment content and photosynthetic performance of P.tricornutum under different Cu^(2+) concentrations(0.1,0.25,0.5 mgL^(-1)Cu^(2+) )were measured.The results showed that theμ,photosynthetic pigment(chlorophyll A and C)content,maximum photochemical quantum yield(Fv/Fm),maximum relative electron transfer rate(rETR_(max)),energy captured per unit reaction center for electron transfer(ETo/RC)and energy captured per unit reaction center for electron transfer to the PSI electron acceptor side(REo/RC)were inhibited by 0.5 mgL^(-1) Cu^(2+) under 400μatm CO_(2) condition,respectively.These parameters were significantly lower than those at 1,500μatm CO_(2)(p<0.05),respectively.Our findings suggested that high concentrations of Cu^(2+) not only restricted the growth of P.trichodensis but also inhibited the activity of photosystem II(PSII)while ocean acidification alleviated the these inhibitions.In the coastal waters where ocean acidification and heavy metal pollution are more serious,ocean acidification could improve the tolerance of P.tricornutum to Cu^(2+) and assist it to survive the complex and changeable environment.

关 键 词：电子传递速率 重金属污染 海洋酸化 光合作用 三角褐指藻 

分 类 号：Q89[生物学]