血清miR-370-3p和miR-152-5p表达与卵巢癌患者临床病理特征及预后的关系  

Relationship between serum miR-370-3p and miR-152-5p expressions and clinical pathological characteristics and prognosis of ovarian cancer patients

作　　者：贾凌彦 谢珊莉 陈泽[2] JIA Lingyan;XIE Shanli;CHEN Ze(Department of Obstetrics and Gynecology,Guangyuan First People's Hospital,Guangyuan 628000,Sichuan,China;Department of Obstetrics and Gynecology,Suining Hospital of Traditional Chinese Medicine,Suining 629099,Sichuan,China)

机构地区：[1]广元市第一人民医院妇产科,四川广元628000 [2]遂宁市中医院妇产科,四川遂宁629099

出　　处：《检验医学》2025年第3期241-246,共6页Laboratory Medicine

基　　金：超声医学工程国家重点实验室开放课题(2021KFKT012)。

摘　　要：目的探讨血清miR-370-3p和miR-152-5p表达与卵巢癌患者临床病理特征及预后的关系。方法选取2018年10月—2020年10月广元市第一人民医院卵巢癌患者108例(卵巢癌组)、女性健康体检者108名(正常对照组)。收集所有研究对象的临床资料,并检测血清miR-370-3p和miR-152-5p表达。对所有卵巢癌患者随访36个月,根据预后情况分为存活组(76例)和死亡组(32例)。采用受试者工作特征(ROC)曲线评价miR-370-3p和miR-152-5p判断卵巢癌患者死亡的效能。采用Kaplan-Meier生存曲线分析卵巢癌患者的生存情况。采用Cox回归分析评估卵巢癌患者死亡的影响因素。结果卵巢癌组血清miR-370-3p、miR-152-5p相对表达量低于正常对照组(P<0.001)。以相对表达量均值确定高表达和低表达,血清miR-370-3p、miR-152-5p高表达组(分别为≥0.75、≥0.76)与低表达组(分别为<0.75、<0.76)之间国际妇产科联合会(FIGO)分期、病理类型、分化程度、淋巴转移、腹水差异均有统计学意义(P<0.05),年龄和肿瘤直径差异均无统计学意义(P>0.05)。死亡组血清miR-370-3p、miR-152-5p相对表达量低于存活组(P<0.001)。ROC曲线分析结果显示,miR-370-3p、miR-152-5p单项检测和联合检测判断卵巢癌患者死亡的曲线下面积(AUC)分别为0.780、0.779、0.897。依据ROC曲线得出的最佳临界值确定,miR-370-3p高表达(≥0.73)组和miR-152-5p高表达(≥0.70)组生存率分别高于miR-370-3p低表达(<0.73)组和miR-152-5p低表达(<0.70)组(Log-rankχ^(2)值分别为13.149、8.554,P值分别为0.001、0.003)。多因素Cox回归分析结果显示,miR-370-3p降低、miR-152-5p降低均是卵巢癌患者死亡的危险因素[风险比(HR)值分别为0.883、0.806,95%可信区间(CI)分别为0.829~0.940、0.714~0.910,P<0.001]。结论卵巢癌患者血清miR-370-3p和miR-152-5p均呈低表达,且与预后密切相关,或可作为卵巢癌患者预后的评估指标。Objective To investigate the relationship between serum miR-370-3p and miR-152-5p expressions and clinical pathological characteristics and prognosis of ovarian cancer patients.Methods From October 2018 to October 2020,108 patients with ovarian cancer and 108 female healthy subjects in Guangyuan First People's Hospital were enrolled.The clinical data of all the subjects were collected,and the expressions of serum miR-370-3p and miR-152-5p were determined.All the patients with ovarian cancer were followed up for 36 months.According to the prognosis,they were classified into survival group(76 cases)and death group(32 cases).Receiver operating characteristic(ROC)curve was used to evaluate the efficacy of miR-370-3p and miR-152-5p in judging the death of ovarian cancer patients.Kaplan-Meier survival curve was used to analyze the survival of patients with ovarian cancer.Cox regression analysis was used to evaluate the prognostic factors of ovarian cancer patients.Results The relative expression levels of serum miR-370-3p and miR-152-5p in ovarian cancer group were lower than those in healthy control group(P<0.001).There was statistical significance in the International Federation of Gynecology and Obstetrics(FIGO)stage,pathological type,degree of differentiation,lymphatic metastasis and ascites between serum miR-370-3p and miR-152-5p high expression groups(≥0.75 and≥0.76)and low expression groups(<0.75 and<0.76)(P<0.05),and there was no statistical significance for age and tumor diameter(P>0.05).The relative expression levels of serum miR-370-3p and miR-152-5p in death group were lower than those in survival group(P<0.001).The areas under curves(AUC)of miR-370-3p,miR-152-5p single determinations and combined determination in judging the death of ovarian cancer patients were 0.780,0.779 and 0.897,respectively.According to the optimal cut-off value obtained by ROC curve,high expression and low expression were distinguished.The survival rates of miR-370-3p and miR-152-5p high expression groups(≥0.73 and≥0.70)were

关 键 词：miR-370-3p miR-152-5p 卵巢癌 临床病理特征 预后 

分 类 号：R446.7[医药卫生—诊断学]