基于转录组测序的玉米瘤黑粉菌UM01240过表达菌株诱导玉米抗病性分析  

Analysis of Disease Resistance Induced by Ustilago maydis Strain with Overexpressed UM01240 Based on Transcriptome Sequencing

作　　者：邹晓威[1] 夏蕾[1] 朱晓敏[1] 孙辉[1] 周琦[3] 齐霁 张亚封 郑岩[1] 姜兆远[1] ZOU XiaoWei;XIA Lei;ZHU XiaoMin;SUN Hui;ZHOU Qi;QI Ji;ZHANG YaFeng;ZHENG Yan;JIANG ZhaoYuan(Institute of Plant Protection,Jilin Academy of Agricultural Sciences(Northeast Agricultural Research Center of China)/Jilin Key Laboratory of Agricultural Microbiology/Key Laboratory of Integrated Pest Management on Crops in Northeast China,Ministry of Agriculture and Rural Affairs,Gongzhuling 136100,Jilin;School of Life Sciences,Jilin Normal University,Siping 136099,Jilin;Agro-Tech Extension and Service Center of Jingyu County,Baishan 135200,Jilin;Faculty of Agronomy,Jilin Agricultural University,Changchun 130118)

机构地区：[1]吉林省农业科学院(中国农业科技东北创新中心)植物保护研究所/吉林省农业微生物重点实验室/农业农村部东北作物有害生物综合治理重点实验室,吉林公主岭136100 [2]吉林师范大学生命科学学院,吉林四平136099 [3]靖宇县农业技术推广中心,吉林白山135200 [4]吉林农业大学农学院,长春130118

出　　处：《中国农业科学》2025年第6期1116-1130,共15页Scientia Agricultura Sinica

基　　金：吉林省农业科技创新项目(KYJF2021ZR105);国家重点研发计划(2023YFD1401504)。

摘　　要：【目的】探究玉米瘤黑粉菌(Ustilago maydis)外分泌蛋白基因UM01240诱导玉米抗瘤黑粉病机制。【方法】将玉米瘤黑粉菌野生型菌株SG200、UM01240过表达菌株SG200-OE-UM01240及UM01240缺失菌株SG200ΔUM01240分别接种于高感瘤黑粉病玉米品种吉单209,接种样品分别命名为S1、S2及S3,7 d后统计供试菌株对玉米致病力差异。利用高通量RNA-Seq技术比较S2玉米叶片与S1、S3玉米叶片之间的差异表达基因,从植物-病原互作、黄酮类化合物生物合成、苯丙烷代谢及植物激素信号转导途径中筛选抗病相关的基因,并进行qRT-PCR验证。【结果】过表达菌株SG200-OE-UM01240相较SG200菌株致病力明显减弱,其对应S2玉米叶片病症最轻,而SG200ΔUM01240致病力较SG200菌株显著增强,其对应S3玉米叶片病症最重。S2玉米叶片相较S1、S3玉米叶片共表达差异基因1 613个,涉及植物-病原互作途径相关基因31个,主要为WRKY1、WRKY33、WRKY52、PR1及PIT5等蛋白基因;黄酮类化合物生物合成途径基因15个,主要为乔松素、柚皮素、木犀草素及二氢槲皮素等合成相关基因;苯丙烷代谢途径基因23个,主要为木质素、肉桂醛、对-香豆醛、松柏醛及芥子醛等合成相关基因;植物激素信号转导途径基因33个,主要为水杨酸、细胞分裂素、赤霉素及茉莉酸等合成相关基因。所选基因qRT-PCR分析结果与转录组数据相关性一致。【结论】外分泌蛋白基因UM01240在玉米瘤黑粉菌致病力中起着重要作用,其表达水平与玉米瘤黑粉菌的致病力负相关。UM01240可能通过调控植物-病原互作、类黄酮和苯丙素类化合物生物合成、植物激素信号转导等途径达到玉米瘤黑粉菌与玉米寄主平衡共生的目的。【Objective】The objective of this study is to investigate the mechanism of maize resistance to corn smut disease induced by the secreted protein gene UM01240 of Ustilago maydis.【Method】The wild-type strain SG200 of U.maydis,the UM01240 overexpression strain SG200-OE-UM01240,and the UM01240 deletion strain SG200ΔUM01240 were individually inoculated onto the maize variety Jidan209,which is highly susceptible to U.maydis.The three inoculated maize samples were named as S1,S2,and S3,respectively.After 7 d of inoculation,the pathogenicity differences among the tested strains on maize were evaluated.Using high-throughput RNA-Seq technology,the differential expression of genes between the S2 maize leaves and the S1 and S3 maize leaves was compared.Resistance-related genes involved in plant-pathogen interactions,flavonoid biosynthesis,phenylpropane metabolism,and plant hormone signal pathways were screened and subsequently validated through qRT-PCR.【Result】The overexpression strain SG200-OE-UM01240 exhibited significantly reduced pathogenicity compared to the SG200 strain,with the corresponding maize sample S2 displaying the mildest leaf symptoms.In contrast,the SG200ΔUM01240 strain demonstrated significantly increased pathogenicity,with the maize sample S3 displaying the most severe leaf symptoms.In comparison to the S1 and S3 maize leaves,the S2 maize leaves exhibited 1613 differentially expressed genes.Among these genes,31 were associated with the plant-pathogen interaction pathway,primarily including protein-coding genes such as WRKY1,WRKY33,WRKY52,PR1,and PIT5.Additionally,15 genes were linked to the flavonoid biosynthesis pathway,primarily including the synthesis of pinocembrin,naringenin,luteolin,and dihydroquercetin.Furthermore,23 genes related to the phenylpropane metabolism pathway were identified,mainly associated with the synthesis of lignin,cinnamaldehyde,p-hydroxycinnamic acid,coniferylaldehyde,and sinapaldehyde.Lastly,33 genes connected to plant hormone signal transduction pathways were also f

关 键 词：玉米瘤黑粉菌 玉米 抗病基因 转录组测序 

分 类 号：S51[农业科学—作物学]