金线莲中金线莲苷及其差向异构体和内源激素测定  

Detection of kinsenoside and it’s isomer,endogenous hormones in Anoectochilus roxburghii

作　　者：李璐璐 朱志彬 李水坤[2] 方洁云 白岩[1] 方莉 钭凌娟 周小军 王红珍[1] LI Lulu;ZHU Zhibin;LI Shuikun;FANG Jieyun;BAI Yan;FANG Li;TOU Lingjuan;ZHOU Xiaojun;WANG Hongzhen(Zhejiang Key Laboratory for Chinese Medicinal Resources Protection and Innovative Utilization,College of Food and Health,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Jiande Forestry Bureau,Jiande 311600,Zhejiang,China;College of Tropical Agriculture and Forestry,Hainan University,Haikou 570204,Hainan,China;Jinhua Academy of Agricultural Science,Jinhua 321017,Zhejiang,China)

机构地区：[1]浙江农林大学食品与健康学院浙江省特色中药资源保护与创新利用重点实验室,浙江杭州311300 [2]建德市林业局,浙江建德311600 [3]海南大学热带农林学院,海南海口570204 [4]金华市农业科学研究院,浙江金华321017

出　　处：《浙江农林大学学报》2025年第2期230-238,共9页Journal of Zhejiang A&F University

基　　金：浙江省基础公益研究计划项目(LGG22H280001);国家林业和草原局科技发展中心生物安全与遗传资源管理专项经费项目(KJZXSA202306);金华市重大科技计划项目(2022-2-009);金华市院地合作项目(2020-J-002-ydhz)。

摘　　要：【目的】金线莲为兰科Orchidaceae植物金线兰Anoectochilus roxburghii新鲜或干燥全草,金线莲苷是其特征性活性成分,金线莲苷差向异构体是其制备过程中极难去除的杂质,本研究旨在明晰金线兰中金线莲苷及其差向异构体、内源激素的含量及分布模式,以筛选金线莲苷工业化生产优质药源。【方法】利用液相色谱-蒸发光散射(HPLC-ELSD)测定金线兰4个品系全草、根、茎和叶中金线莲苷及其差向异构体质量分数,并分析其分布模式;利用液相色谱中二极阵列检测器(HPLC-PDA)测定金线兰不同部位茎段、叶片中内源生长素(IAA)、水杨酸(SA)和脱落酸(ABA)质量分数,分析其与金线莲苷积累模式相关性。【结果】金线兰4个品系全草中,金线莲苷质量分数有所不同,范围为2.84~3.81 mg·g^(-1),从大到小依次为‘大叶’‘Daye’、‘小叶’‘Xiaoye’、‘尖叶’‘Jianye’和‘红霞’‘Hongxia’,地上部分组织中金线莲苷质量分数远高于根,并且叶中略高于茎;金线兰4个品系中金线莲苷差向异构体质量分数极低,为0.14~0.50 mg·g^(-1),在根部特异性积累;在红霞类原球茎中同时检测到金线莲苷及其差向异构体,其金线莲苷质量分数几乎与全草相当;在尖叶类原球茎中只检测到金线莲苷,其质量分数高于全草。金线莲苷和IAA积累模式在金线兰4个品系茎段中正相关,金线莲苷与ABA积累模式只在红霞和小叶品系茎段中正相关;金线莲苷与SA积累模式在‘小叶’‘尖叶’‘红霞’品系茎段中显著正相关(P<0.05)。金线兰经生长素抑制剂(TIBA)处理前后,金线莲苷积累模式随着IAA积累模式变化而变化,金线莲苷合成或受内源IAA合成调控。【结论】明确了金线兰中金线莲苷及其差向异构体的分布模式,金线莲苷特异性分布在金线兰的地上部分,金线莲苷差向异构体特异性分布于根部,尖叶类原球茎中没有检测到金线莲苷�[Objective]Kinsenoside is a characteristic active ingredient of Anoectochilus roxburghii,and it’s isomer is impurity that is extremely difficult to remove during kinsenoside preparation process.[Method]Kinsenoside and it’s isomer contents in whole plant,root,stem and leaf of‘Jianye’,‘Hongxia’,‘Daye’,and‘Xiaoye’cultivars were detected using HPLC-ELSD,and their accumulation pattern were also analyzed.The contents of endogenous IAA,ABA,and SA were detected using HPLC-PDA,and correlation coefficient of kinsenoside and these hormones accumulation pattern were also analyzed using Pearson correlation analysis.[Result]Kinsenoside contents(2.84 to 3.81 mg·g^(-1))were varied in 4 cultivars of A.roxburghii,and that was the highest in‘Daye’,moderate in‘Xiaoye’and‘Jianye’,and the lowest in‘Hongxia’.Kinsenoside accumulated predominantly in aerial parts,and it’s content was slighter higher than that in stem.Meanwhile,the content of it’s isomer were very low in all cultivars(0.14 to 0.50 mg·g^(-1))and it exclusively accumulated in root.Kinsenoside and it’s isomer simultaneously detected in the protocorm like bodies(PLBs)of‘Hongxia’,and kinsenoside content was almost equal to that of the whole plant.Kinsenoside was detected in PLBs in‘Jianye’,and it’s content was higher than that whole plant,while there is no it’s isomer was detected in PLBs of‘Jianye’.Kinsenoside and IAA in a polar manner in the stem of all 4 cultivars were positive correlated.Meanwhile kinsenoside and SA accumulation pattern were positively correlated‘Xiaoye’,‘Hongxia’and‘Jianye’cultivars.Kinsenoside accumulation pattern hanged with the IAA accumulation pattern after TIBA treatment.These results showed that kinsenoside biosynthesis may be regulated by endogenous IAA accumulation.[Conclusion]This study identified the distribution patterns of kinsenoside and it’s isomer in A.roxburghii.Kinsenoside specificity was distributed in the aboveground part,while the isomer was distributed in the ro

关 键 词：金线兰 金线莲苷及其差向异构体 分布模式 内源生长激素 生长素抑制剂 

分 类 号：S759.8[农业科学—森林经理学] Q946.8[农业科学—林学]