2种枳椇属植物果梗的广泛靶向代谢组分析  

Comprehensive targeted metabolomic analysis of fruit stalks in two species of Hovenia plants

作　　者：应俊辉[1] 潘温文 张华[2] 周生财 林艳 张俊红[2] 陈世通 YING Junhui;PAN Wenwen;ZHANG Hua;ZHOU Shengcai;LIN Yan;ZHANG Junhong;CHEN Shitong(Lishui Vocational&Technical College,Lishui 323000,Zhejiang,China;National Key Laboratory for Development and Utilization of Forest Food Resources,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Baiyun Mountain Ecological Forest Farm in Lishui City,Lishui 323000,Zhejiang,China;Lishui Forestry Bureau,Lishui 323000,Zhejiang,China)

机构地区：[1]丽水职业技术学院,浙江丽水323000 [2]浙江农林大学森林食物资源挖掘与利用全国重点实验室,浙江杭州311300 [3]丽水市白云山生态林场,浙江丽水323000 [4]丽水市林业局,浙江丽水323000

出　　处：《浙江农林大学学报》2025年第2期348-356,共9页Journal of Zhejiang A&F University

基　　金：2023浙江省林业专项(浙林规[2023]10号)。

摘　　要：【目的】阐明枳椇Hovenia acerba和光叶毛果枳椇H. trichocarpa var. robusta果梗的代谢物差异,为枳椇属Hovenia植物的开发利用奠定基础。【方法】通过高效液相色谱-串联质谱(HPLC-MS/MS)的广泛靶向代谢组学方法,分析枳椇和光叶毛果枳椇果梗中代谢产物和代谢通路的差异。【结果】2种枳椇属植物果梗中共检测到1 810种代谢物,其中显著差异代谢物782种(P<0.05)。与枳椇果梗相比,光叶毛果枳椇果梗中上调差异代谢物有378种,下调差异代谢物有404种,且单宁、醌类代谢物相对含量均显著下调(P<0.05)。2种枳椇属植物果梗中差异代谢物主要富集在苯丙烷类化合物、黄酮类化合物和异喹啉生物碱的生物合成途径,其中极显著差异代谢物包含乌药碱、N-甲基乌药碱和甲基衡州乌药碱等生物碱物质以及柚皮素、柚皮素查尔酮和2’, 3, 4, 4’, 6’-五羟基查耳酮等黄酮类产物。【结论】同一生态条件下,2种枳椇果梗的代谢谱存在显著差异,特别是具有药用价值和高甜度的生物碱和黄酮类代谢产物相对含量存在显著差异。[Objective]This study aims to clarify the metabolite differences in the fruit stalks of Hovenia acerba and H.trichocarpavar var.robusta,and lays a foundation for the development and utilization of Hovenia plants.[Method]Through a comprehensive targeted metabolomics approach of high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),the differences in metabolites and metabolic pathways in the fruit stalks of H.acerba and H.trichocarpa var.robusta were analyzed.[Result]A total of 1810 metabolites were detected in the fruit stalks of the two Hovenia species,of which 782 metabolites showed significant differences(P<0.05).Compared with the fruit stalks of H.acerba,there were 378 upregulated differential metabolites and 404 down-regulated differential metabolites in the fruit stalks of H.trichocarpa var.robusta,and the relative contents of tannin and quinone metabolites were significantly downregulated.The differential metabolites in the fruit stalks of the two Hovenia species were primarily enriched in the biosynthetic pathways of phenylalanine compounds, flavonoids, and isoquinoline alkaloids. Among them, the extremely significant differential metabolites included alkaloid substances such as ligustroside, N-methylligustroside and methylcoclaurine, as well as flavonoids like naringin, naringin chalcone, and 2’, 3, 4, 4’, 6’-pentahydroxy chalcone. [Conclusion] There are significant differences in the metabolic profiles of the fruit stalks of the two Hovenia species under the same ecological conditions, especially the relative content of alkaloids and flavonoids with medicinal value and high sweetness.

关 键 词：枳椇属 广靶代谢组 果梗 生物碱 黄酮化合物 

分 类 号：S722[农业科学—林木遗传育种]