全自动生化分析仪测定水中氟化物的方法研究  

A method for determining fluoride in water using a fully automated biochemical analyzer

作　　者：刘婷婷[1] 王开倩 张俊华 胡越 谢继安[1] 丁刚[1] 李卫东[1] 徐署东 Liu Tingting;Wang Kaiqian;Zhang Junhua;Hu Yue;Xie Ji'an;Ding Gang;Li Weidong;Xu Shudong(Physical and Chemical Laboratory,Anhui Center for Disease Control and Prevention,Hefei 230601,China)

机构地区：[1]安徽省疾病预防控制中心理化检验室,合肥230601

出　　处：《中华地方病学杂志》2025年第2期142-145,共4页Chinese Journal of Endemiology

摘　　要：目的建立一种全自动生化分析仪测定水中氟化物的方法(简称本方法)。方法结合全自动生化分析仪参数,设置合适的各试剂体积和反应时间,在标准曲线范围(0.0~2.0 mg/L)内测定水中氟离子。从本方法的线性范围、检出限、精密度、准确度等方面进行效果验证,并与《生活饮用水标准检验方法第5部分:无机非金属指标》(GB/T 5750.5—2023)中的氟试剂分光光度法手动测定结果进行方法对比实验。结果在0.0~2.0 mg/L氟质量浓度范围内,本方法相关系数的绝对值均>0.9990,检出限为0.082 mg/L;低、中、高3种氟质量浓度水样测定氟含量的相对标准偏差分别为4.03%、2.16%、1.68%;加标回收率分别为98.1%、99.5%、100.2%;本方法与手动测定结果比较,差异无统计学意义(t=1.07,P=0.295)。结论本方法检测氟化物灵敏度高、准确性好、效率高,仪器设备代替手动操作,可实现全自动样品的快速检测。ObjectiveTo establish a method for determination of fluoride in water using a fully automated biochemical analyzer(abbreviated as this method).MethodsBased on the parameters of the fully automatic biochemical analyzer,appropriate reagent volumes and reaction time were optimized to determine fluoride ions in water within the standard curve range(0.0-2.0 mg/L).The method was validated through evaluation of linear range,detection limit,precision,accuracy,and comparative analysis with the manual measurement results of fluoride reagent spectrophotometric method specified in the"Standard Examination Methods for Drinking Water-Part 5:Inorganic Nonmetallic indices"(GB/T 5750.5-2023).ResultsWithin the range of 0.0-2.0 mg/L fluoride mass concentration,the absolute values of the correlation coefficients were>0.9990 and the detection limit of this method was 0.082 mg/L.The relative standard deviation for the determination of fluoride level in water samples with low,medium and high fluoride mass concentrations was 4.03%,2.16%and 1.68%,respectively.The spiked recovery rates were 98.1%,99.5%,and 100.2%,respectively.There was no statistically significant difference between the results obtained by this method and manual measurement(t=1.07,P=0.295).ConclusionThis method exhibits high sensitivity,accuracy,and efficiency in detecting fluoride,enabling rapid sample detection through instrumental automation instead of manual operation.

关 键 词：氟化物 全自动生化分析仪 自动检测 高效 

分 类 号：O65[理学—分析化学]