抗逆转录病毒治疗对HIV-1感染者PD-1^(+)CD160^(+)CD3^(+)T细胞的影响  

The Impact of Antiretroviral Therapy on PD-1^(+)CD160^(+)CD3^(+)T Cells in People Living with HIV-1

作　　者：彭丽珊[1] 杨洋[1] 伍月榕 刘显[1] PENG Lishan;YANG Yang;WU Yuerong;LIU Xian(Guangxi Key Laboratory of Translational Medicine of Integrated Traditional Chinese and Western Medicine,Guangxi University of Traditional Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学广西高发传染病中西医结合转化医学重点实验室,广西南宁530200

出　　处：《标记免疫分析与临床》2025年第2期303-308,共6页Labeled Immunoassays and Clinical Medicine

基　　金：国家自然科学基金(编号:81560547);广西高校中青年教师基础研究能力提升项目(编号:2021KY0280)。

摘　　要：目的探讨抗逆转录病毒治疗(antiretroviral treatment,ART)对HIV-1感染者(people living with HIV-1,PLWH)CD3^(+)T细胞PD-1和CD160表达及其活化凋亡水平的影响。方法收集66例慢性HIV-1感染患者,根据是否接受ART治疗,将PLWH分为non-ART组(39例)和ART组(27例),并设置HIV-1阴性对照组(30例)。采用流式细胞术检测3组外周血CD4计数及CD3^(+)T细胞中PD-1、CD160、CD95、CD38和HLA-DR的表达,并分析3组间不同指标的差异性。结果non-ART组CD3^(+)T细胞百分比和PD-1^(+)T细胞百分比与ART组相比差异无统计学意义(P=0.8141,P>0.9999),而其CD160^(+)CD3^(+)T细胞百分比显著高于ART组(P=0.0162)。共表达分析显示,non-ART组PD-1^(+)CD160^(+)CD3^(+)T细胞百分比显著高于ART组(P=0.0121),而在单阳的CD3^(+)T性细胞(CD160^(+)PD-1^(-)和PD-1^(+)CD160-)细胞群,ART组和non-ART组差异均无统计学意义(P=0.3382,P>0.9999)。在PD-1^(+)CD160^(+)CD3^(+)T细胞CD38方面,non-ART组显著高于ART组和HIV-1阴性对照组(P=0.0005,P=0.0015),HIV-1阴性对照组和ART组差异无统计学意义(P>0.9999)。结论ART对PLWH中PD-1^(+)CD160^(+)CD3^(+)T细胞百分比及其CD38的表达具有调节作用。Objective This study aims to investigate the impact of antiretroviral therapy(ART)on the expression and activation depletion levels of PD-1 and CD160 on CD3^(+)T cells in people living with HIV-1(PLWH).Methods A total of 66 patients with chronic HIV-1 infection were recruited and then categorized into two case groups:a non-ART group consisting of 39 individuals who had not received ART treatment,and an ART group comprising 27 individuals who had undergone ART treatment.Additionally,an HIV-1 negative control group of 30 individuals was established.Flow cytometry was employed to measure the peripheral blood CD4 count and the expression levels of PD-1,CD160,CD95,CD38,and HLA-DR on CD3^(+)T cells across the three groups.The differences of these indicators among the three groups were statistically analyzed.Results In comparing the non-ART and ART groups,no significant differences were observed in the percentage of CD3^(+)T cells and PD-1^(+)T cells(P=0.8141,P>0.9999).However,the percentage of CD160^(+)CD3^(+)T cells was significantly higher in the non-ART group compared with the ART group(P=0.0162).Co-expression analysis revealed that the percentage of PD-1^(+)CD160^(+)CD3^(+)T cells was significantly elevated in the non-ART group relative to the ART group(P=0.0121).In contrast,there were no significant differences between the ART and non-ART groups in the populations of single-positive CD3^(+)T cells,specifically CD160^(+)PD-1^(-)and PD-1^(+)CD160-cells(P=0.3382,P>0.9999).Regarding CD38 expression on PD-1^(+)CD160^(+)CD3^(+)T cells,the non-ART group exhibited significantly higher levels than both the ART group and the HIV-1 negative control group(P=0.0005,P=0.0015),while no significant difference was found between the HIV-1 negative control group and the ART group(P>0.9999).Conclusion ART shows an impact on the percentage of PD-1^(+)CD160^(+)CD3^(+)T cells and the expression of CD38 in PLWH.

关 键 词：ART HIV-1 CD3^(+)T细胞 PD-1 CD160 

分 类 号：R512.91[医药卫生—内科学]