咖啡酸调节TLR4/NF-κB信号通路对呼吸道合胞病毒感染小鼠肺组织损伤的影响  

The Effect of Caffeic Acid on Lung Tissue Damage in Mice Infected with Respiratory Syncytial Virus by Regulating the TLR4/NF-κB Signaling Pathway

作　　者：马玲彦 宋冬青 葛胜华 MA Lingyan;SONG Dongqing;GE Shenghua(Department of pediatrics,Shijiazhuang People’s Hospital,Shijiazhuang 050000,China)

机构地区：[1]石家庄市人民医院儿科,河北石家庄050000

出　　处：《标记免疫分析与临床》2025年第2期387-393,共7页Labeled Immunoassays and Clinical Medicine

摘　　要：目的研究咖啡酸调节TLR4/NF-κB信号通路对呼吸道合胞病毒(RSV)感染小鼠肺组织损伤的影响。方法取C57BL/6小鼠随机分为对照组、模型组、咖啡酸低剂量(30mg/kg)组、咖啡酸高剂量(60mg/kg)组、咖啡酸高剂量(60mg/kg)+脂多糖(LPS)(TLR4激活剂,2.5mg/kg)组,模型组与药物干预组小鼠以RSV滴鼻感染法建立感染模型,对照组小鼠鼻腔滴入等量生理盐水,以咖啡酸和LPS分组干预治疗后检测小鼠肺功能,比较各组呼气峰流速(PEF)、用力肺活量(FVC)、气道阻力(Raw);检测各组小鼠肺泡灌洗液(BALF)中淋巴细胞数与肺含水量;以HE染色检测小鼠肺组织病理变化,比较各组肺组织病理评分;以ELISA检测各组小鼠血清与BALF促炎因子水平;以免疫印迹检测各组小鼠肺组织TLR4/NF-κB信号通路蛋白表达。结果与对照组相比,模型组小鼠肺组织发生明显病理损伤,FVC、PEF降低(P<0.05),Raw、BALF中炎性细胞数、肺含水量、肺组织病理评分、BALF与血清IL-1β、干扰素γ(IFN-γ)、IL-6水平、TLR4蛋白表达与p-NF-κB p65/NF-κB p65显著升高(P<0.05);与模型组相比,咖啡酸低剂量组、咖啡酸高剂量组小鼠肺组织损伤减轻,FVC、PEF升高(P<0.05),Raw、BALF中淋巴细胞数、肺含水量、肺组织病理评分、BALF与血清IL-1β、干扰素γ(IFN-γ)、IL-6水平、TLR4蛋白表达与p-NF-κB p65/NF-κB p65降低(P<0.05);与咖啡酸低剂量组相比,咖啡酸高剂量组小鼠肺组织损伤减轻,FVC、PEF升高(P<0.05),Raw、BALF中淋巴细胞数、肺含水量、肺组织病理评分、BALF与血清IL-1β、干扰素γ(IFN-γ)、IL-6水平、TLR4蛋白表达与p-NF-κB p65/NF-κB p65降低(P<0.05);与咖啡酸高剂量组相比,咖啡酸高剂量+LPS组小鼠肺组织损伤加重,FVC、PEF降低(P<0.05),Raw、BALF中淋巴细胞数、肺含水量、肺组织病理评分、BALF与血清IL-1β、干扰素γ(IFN-γ)、IL-6水平、TLR4蛋白表达与p-NF-κB p65/NF-κB p65升高(P<0.05)。结论咖啡酸可�Objective To investigate the effect of caffeic acid on lung tissue damage in mice infected with Respiratory syncytial virus(RSV)by regulating the TLR4/NF-κB signaling pathway.Methods C57BL/6 mice were randomly grouped into a control group,a model group,a low-dose(30mg/kg)caffeic acid group,a high-dose(60mg/kg)caffeic acid group,and a high-dose(60mg/kg)caffeic acid+lipopolysaccharide group(LPS)(TLR4 activator,2.5mg/kg)group.The mice of the model group and drug intervention group were infected by RSV nasal drip infection method.The mice of the control group were given an equal amount of physiological saline via nasal drip.Their lung function was measured after intervention treatment with caffeic acid and LPS.The peak expiratory flow rate(PEF),forced vital capacity(FVC),and airway resistance(Raw)of each group were compared.The lymphocyte count and lung water content in bronchoalveolar lavage fluid(BALF)of mice in each group were detected.HE staining was applied to detect pathological changes in mouse lung tissue,and the pathological scores of lung tissue in each group were compared.ELISA was applied to detect the levels of serum and BALF pro-inflammatory factors of mice in each group.Immunoblotting was applied to detect the expression of TLR4/NF-κB signaling pathway proteins in lung tissue of mice in each group.Results Compared with the control group,the lung tissue of mice in the model group showed significant pathological damage,while the FVC and PEF decreased significantly(P<0.05);The Raw,lymphocyte count in BALF,lung water content,lung tissue pathological score,the levels of IL-1β,interferon-γ(IFN-γ),and IL-6 in BALF and serum,the expression of TLR4 protein,and p-NF-κB p65/NF-κB p65 increased significantly(P<0.05).Compared with the model group,the lung tissue damage in the low-dose and high-dose caffeic acid groups was reduced,while the FVC and PEF increased significantly(P<0.05);the Raw,lymphocyte count in BALF,lung water content,lung tissue pathological score,the levels of IL-1β,interferon-γ(IFN-γ),a

关 键 词：咖啡酸 TLR4/NF-κB 呼吸道合胞病毒 感染 肺组织损伤 

分 类 号：R511[医药卫生—内科学]