Elecsys HBsAg II检测HBV感染者血清HBsAg变异性分析  

An Analysis of Serum HBsAg Variability in HBV Infected Persons by Elecsys HBsAg II Assay

作　　者：温惠萍[1] 吴茗 刘洋[1] 蒋秀娣[1] 万维琴 何成山 WEN Huiping;WU Ming;LIU Yang;JIANG Xiudi;WAN Weiqin;HE Chengshan(Department of Clinical Laboratory,The Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China;Department of Clinical Laboratory,Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University,Shanghai 201799,China;Outpatient Department of the 95851 Unit of theChinese People’s Liberation Army,Shanghai 200137,China)

机构地区：[1]上海中医药大学附属第七人民医院医学检验科,上海200137 [2]复旦大学附属中山医院青浦分院医学检验科,上海201799 [3]中国人民解放军95851部队门诊部,上海200137

出　　处：《标记免疫分析与临床》2025年第2期394-400,共7页Labeled Immunoassays and Clinical Medicine

基　　金：上海市浦东新区卫生健康委员会科研面上项目(编号:PW2021A-18);上海市浦东新区卫生健康委员会薄弱学科建设项目:检验与病理学(编号:PWZbr2022-08)。

摘　　要：目的了解区域内乙型肝炎病毒(HBV)流行基因型及乙型肝炎病毒表面抗原(HBsAg)变异情况,评价Elecsys HBsAg II可识别的HBsAg变异类型。方法收集HBsAg和HBV DNA阳性样本,提取血清基因组DNA,采用自建PCR体系扩增HBV S区基因,经琼脂糖凝胶电泳观察目的基因条带,切割目的条带测序,测序结果与标准野生型序列比对。结果150例样本成功扩增出HBV S区基因,HBsAg变异率达87.33%(131/150),检出5种HBV基因亚型,其中B基因型1种(58株),C基因型4种(92株),C基因型为流行优势感染株;HBV S区基因99个位点发生144种不同类型共计640次氨基酸突变,突变率1.82%(640/33900),各亚型在HBV S区基因不同表位区域突变率均存在较大差异(P<0.01),其中“α”决定簇内检出R122K、K122R、I126S/T、T126S/A、S126I、P127S/T、Q129H、M133T/I、F134L和S143T等14种不同类型的65次氨基酸突变。结论HBV感染者血清HBsAg变异性复杂,Elecsys HBsAg II可有效识别多种HBsAg变异类型,适用于HBsAg的筛查诊断。Objective To investigate the endemic genotype of hepatitis B virus(HBV)and the variation of hepatitis B virus surface antigen(HBsAg)in the region,and to evaluate the HBsAg variation types recognized by Elecsys HBsAg II assay.Methods Samples of HBsAg and HBV DNA positive were collected for the study.Serum genomic DNA was extracted,and the HBV S region gene was amplified by self-built PCR system.The target gene bands were observed by agarose gel electrophoresis,and the target bands were cut and sequenced.The sequencing results were then compared with standard wild type sequences.Results The mutation rate of HBsAg was 87.33%(131/150).Five types of HBV gene subtypes were detected,including 1 type B(58 strains)and 4 types C(92 strains).The C genotype was the predominant infection strain.There were 640 amino acid mutations of 144 different types in 99 sites of HBV S region gene,with a mutation rate of 1.82%(640/33900),and the mutation rates of different subtypes in different epitope regions of HBV S region gene were significantly different(P<0.01).Among them,65 amino acid mutations of 14 different types,such as R122K,K122R,I126S/T,T126S/A,S126I,P127S/T,Q129H,M133T/I,F134L and S143T were detected in the“α”determinant.Conclusion The variability of serum HBsAg in HBV-infected persons shows a complex pattern.Elecsys HBsAg II can effectively identify a variety of HBsAg variants and is suitable for screening and diagnosis purposes of HBsAg.

关 键 词：乙型肝炎病毒 乙型肝炎病毒表面抗原 检测 感染 变异 

分 类 号：R373.21[医药卫生—病原生物学] R512.62[医药卫生—基础医学]