报春石斛的化学成分及其生物活性  

Chemical Composition and Biological Activity of Dendrobium primulinum

作　　者：顾润琦 廖勤昌 周莹 何璨羽 赵雅琪 庄晨曦 李玉鹏 GU Runqi;LIAO Qinchang;ZHOU Ying;HE Canyu;ZHAO Yaqi;ZHUANG Chenxi;LI Yupeng(School of Pharmaceutical Science&Yunnan Key Laboratory of Pharmacology for Natural;School of Modern Biomedicine Industry,Kunming Medical University,Kunming Yunnan 650500;Institute of Caulis Dendrobii of Longling County,Baoshan Yunnan 678300;School of Pharmacy,Honghe Health Vocational College,Honghe Yunnan 661100,China)

机构地区：[1]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500 [2]昆明医科大学现代生物医药产业学院,云南昆明650500 [3]龙陵县石斛研究所,云南保山678300 [4]红河卫生职业学院药学院,云南红河661100

出　　处：《昆明医科大学学报》2025年第3期7-10,共4页Journal of Kunming Medical University

基　　金：国家自然科学基金(21202066);云南省“兴滇英才支持计划”产业创新人才专项(201904);云南省科技厅-昆明医科大学联合专项资助项目(202301AY070001-212);云南省本科教育教学改革研究项目“探索建设示范性现代生物医药产业学院”(JG2023001);云南省教育厅基金资助项目(2024J0149);昆明医科大学2024年度校级大学生创新训练计划项目(2024CYD415)。

摘　　要：目的研究报春石斛化学成分,发现其活性成分。方法报春石斛经溶剂提取、硅胶和凝胶柱色谱分离,波谱分析确定结构。部分化合物体外活性筛选采用MTT法。结果从报春石斛中分离鉴定了7个化合物,分别为7-dihydroxy-2,4-methoxyphenanthrene(1)、densiflorol B(2)、3,4,7-trihydroxy-2-methoxy-9,10-dihydrophenanthrene(3)、thunalbene(4)、phillygenin(5)、3β-hydroxy-5α,8α-epidioxyergosta-6,22-diene(6)、4-hydroxy-3,5-dimethoxycinnamaldehyde(7),对化合物1~5进行抗肿瘤活性测试,结果显示化合物1对MCF-7/S细胞株抑制活性较强。结论化合物1~6为首次从本植物中分离得到,化合物7为首次从本属植物中分离得到,1对MCF-7/S细胞株IC50为5.13μM。Objective To study the chemical composition of and identify its active components.Methods The chemical constituents of Dendrobium Primulinum were extracted using solvents,followed by separation through silica gel and gel column chromatography,with structural identification performed via spectral analysis.The in vitro activity screening of some compounds was conducted using the MTT assay.Results Seven compounds were isolated and identified from Dendrobium primulinum,namely 7-dihydroxy-2,4-methoxyphenanthrene(1),densiflorol B(2),3,4,7-trihydroxy-2-methoxy-9,10-dihydrophenanthrene(3),thunalbene(4),phillygenin(5),3β-hydroxy-5α,8α-epidioxyergosta-6,22-diene(6)、4-hydroxy-3,5-dimethoxy--cinnamaldehyde(7).Antitumor activity tests were performed on compounds 1 to 5,revealing that compound 1 exhibited strong inhibitory activity against the MCF-7/S cell line,with an IC50 of 5.13μM.Conclusion Compounds 1 to 6 are reported for the first time from Dendrobiumprimulinum,while compound 7 is reported for the first time from this genus.

关 键 词：报春石斛 化学成分 生物活性 

分 类 号：R284.2[医药卫生—中药学]