心房颤动患者外周淋巴细胞LINC02009、LOC107984895表达水平的临床研究  

Clinical Study on the Expression Levels of LINC02009 and LOC107984895 in Peripheral Lymphocytes of Patients with Atrial Fibrillation

作　　者：王宇[1] 彭睿[2] 田倪妮 赵晓丽[3] 郑松青[1] 贾永全[1] 苗文清[2] 刘巧梅 WANG Yu;PENG Rui;TIAN Nini;ZHAO Xiaoli;ZHENG Songqing;JIA Yongquan;MIAO Wenqing;LIU Qiaomei(Department of Cardiology,The First People’s Hospital of Kunming,Kunming Yunnan 650224,China;Department of General Practice,The First People’s Hospital of Kunming,Kunming Yunnan 650224,China;Department of Laboratory Medicine,The First People’s Hospital of Kunming,Kunming Yunnan 650224,China)

机构地区：[1]昆明市第一人民医院心内科,云南昆明650224 [2]昆明市第一人民医院全科医学科,云南昆明650224 [3]昆明市第一人民医院检验科,云南昆明650224

出　　处：《昆明医科大学学报》2025年第3期66-71,共6页Journal of Kunming Medical University

基　　金：昆明市卫生健康委员会“十百千”人才培养计划(2022-sw-(后备)-56);昆明市卫生健康委基金(2022-03-01-001);云南省科技厅-昆明医科大学联合专项(202401AY070001-279);昆明市肝病诊治医学技术中心[2022-sw-(技术)-28]。

摘　　要：目的 探讨心房颤动患者外周淋巴细胞中LINC02009、LOC107984895表达水平及临床意义。方法 选择2023年1月至2023年12月昆明市第一人民医院住院的75例房颤患者(其中持续性房颤50例,阵发性房颤25例)为研究对象,同期正常对照患者50例。采用实时定量PCR检测房颤患者外周血白细胞中LINC02009、LOC107984895的表达水平。运用Logistic回归分析评估其表达水平与房颤发生的危险因素及相关性,运用ROC曲线预测LINC02009、LOC107984895诊断房颤的诊断界值。结果 AF(阵发性房颤、持续性房颤)组、Normal组患者在高血压、冠心病等基础疾病中,在Cr、BNP等生化指标中,在LAd、LVEF等心肌重构指标中差异有统计学意义(P <0.05)。房颤(阵发性房颤、持续性房颤)组血浆中LINC02009、LOC107984895表达水平明显高于对照组(P <0.05);与LVEF呈负相关(P <0.05)。LINC02009、LOC107984895预测心房颤动的曲线下面积(area under curve,AUC)分别为0.967(95%CI 0.938~0.995)、0.900 (95%CI 0.838~0.963);最佳截断值为1.985、0.915;敏感度为88%,76%;特异度为94%、90%。结论 LINC02009、LOC107984895为心房颤动的独立危险因素,对房颤发生有一定预测价值。Objective To investigate the expression levels of LINC02009 and LOC107984895 in peripheral lymphocytes of patients with atrial fibrillation and their clinical significance.Methods A total of 75 hospitalized patients with atrial fibrillation(50 with persistent atrial fibrillation and 25 with paroxysmal atrial fibrillation)from Kunming First People’s Hospital between January 2023 and December 2023 were selected as study subjects,along with 50 normal control patients.Real-time quantitative PCR was used to detect the expression levels of LINC02009 and LOC107984895 in peripheral blood leukocytes of patients with atrial fibrillation.Logistic regression analysis was employed to assess the relationship between expression levels and risk factors for atrial fibrillation,and ROC curves were used to predict the diagnostic cut-off values for LINC02009 and LOC107984895 in diagnosing atrial fibrillation.Results There were statistically significant differences in baseline diseases such as hypertension and coronary heart disease,biochemical indicators such as Cr and BNP,and myocardial remodeling indicators such as LAd and LVEF between the AF(paroxysmal atrial fibrillation and persistent atrial fibrillation)group and the Normal group(P<0.05).The expression levels of LINC02009 and LOC107984895 in the plasma of the atrial fibrillation group were significantly higher than those in the control group(P<0.05)and were negatively correlated with LVEF(P<0.05).The areas under the curve(AUC)of LINC02009 and LOC107984895 in predicting atrial fibrillation were 0.967(95%CI:0.938~0.995)and 0.900(95%CI:0.838~0.963),respectively.The optimal cut-off values were 1.985 and 0.915,with sensitivities of 88%and 76%,respectively,and specificities of 94%and 90%,respectively.Conclusion LINC02009 and LOC107984895 are independent risk factors for atrial fibrillation and have certain predictive value for the occurrence of atrial fibrillation.

关 键 词：心房颤动 RNA 长链非编码 

分 类 号：R541.75[医药卫生—心血管疾病]