叶面喷施不同浓度锰对黄芩根生物量和黄酮类化合物含量的影响  

Effects of foliar spraying of different manganese levels on Scutellaria baicalensis root biomass and the synthesis of flavonoids

作　　者：王彦婷 安晓静 李先恩 陈彩霞 马春英[1] WANG Yan-ting;AN Xiao-jing;LI Xian-en;CHEN Cai-xia;MA Chun-ying(College of Agronomy,Hebei Agricultural University,Baoding,Hebei 071000,China;National Engineering Laboratory for Breeding of Endangered Medicinal Materials/Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education/Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;School of Chinese Materia Medica,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]河北农业大学农学院,河北保定071000 [2]中国医学科学院北京协和医学院药用植物研究所/中草药物质基础与资源利用教育部重点实验室/濒危药材繁育国家工程实验室,北京100193 [3]天津中医药大学中药学院,天津301617

出　　处：《植物营养与肥料学报》2025年第3期542-553,共12页Journal of Plant Nutrition and Fertilizers

基　　金：内蒙古自治区科技计划项目(2021GG0385)。

摘　　要：【目的】探明氯化锰不同喷施浓度对黄芩生长及黄酮类化合物合成的影响。【方法】在北京市海淀区进行田间和盆栽(避雨大棚)试验,以1年生黄芩为试验材料,试验设清水对照(Mn0)及0.5 g/L(Mn1)、2 g/L(Mn2)、4 g/L(Mn3)、8 g/L(Mn4)MnCl_(2)溶液等5个处理。在生长旺期(2023−08−07)进行第一次叶面喷肥,每隔15天喷施1次,共喷3次肥。于秋季黄芩成熟时(2023−10−08)采收,测定其根系生长指标、黄酮化合物含量和叶片激素水平及抗氧化酶活性等生理指标。【结果】1)盆栽试验根系生物量和总黄酮产量均随Mn浓度增加呈先升后降再升趋势,在Mn1处理时达到峰值,分别较Mn0处理显著增加了35%、48%。SOD活性随Mn浓度增加呈先升后降再升趋势,在Mn1处理时达到峰值,较Mn0处理显著增高了8%,Mn3处理显著低于Mn0处理21%。与Mn0处理相比,Mn2、Mn3和Mn4处理下POD活性显著下降了2%~13%。IAA含量随Mn浓度增加呈先降后升再降趋势,Mn3、Mn4处理显著低于Mn0处理20%~25%;GA含量随Mn浓度增加呈先升后降趋势,在Mn2处理时达到峰值,较Mn0处理提高了8%,Mn4处理GA含量最低,较Mn0处理下降了4%。ABA含量随Mn浓度增加呈先降后升趋势,Mn4处理较Mn0处理显著提高了13%,Mn2处理较Mn0处理显著降低了6%。2)田间试验根系生物量随Mn浓度增加呈先升后降趋势,在Mn1处理时达到峰值,较Mn0处理显著增加了53%。总黄酮产量随Mn浓度增加呈先升后降趋势,在Mn1处理时达到峰值,较Mn0处理显著增加了53%。SOD随Mn浓度增加呈先升后降再升趋势,除Mn1处理与Mn0处理无显著差异外,Mn2、Mn3、Mn4处理较Mn0处理显著降低了8%~12%。IAA含量随Mn浓度增加呈先升后降趋势,与M0处理相比,Mn1处理显著提高了6%,Mn3、Mn4处理显著降低了11%~15%;GA含量随Mn浓度增加呈先升后降的趋势,在Mn3处理时达到峰值,较Mn0处理提高了15%。ABA含量随Mn浓度增加呈先降后升再降又升的趋势,Mn4处【Objectives】The objective of this study is to investigate the effects of different manganese chloride levels on the growth and flavonoid synthesis of Scutellaria baicalensis.【Methods】Two experiments were carried out in Haidian District,Beijing,using annual skullcap as the test material,and five treatments were set up:clean water control(Mn0),0.5 g/L(Mn1),2 g/L(Mn2),4 g/L(Mn3)and 8 g/L(Mn4)MnCl_(2)solution.The first foliar spray was carried out on August 7 during the peak growth period,and it was sprayed once every 15 days,for a total of 3 sprays.Scutellaria baicalensis was harvested in autumn when it was ripe(October 8),and its root growth indexes,flavonoid compound content,leaf hormone and antioxidant enzyme activities and other physiological indexes were measured.【Results】1)Pot experiment Root biomass and total flavonoid yield increased first and then decreased and then increased again with the increase of Mn concentration,reaching a peak at Mn1 treatment,which was significantly increased by 35%and 48%respectively compared with Mn0.With the increase of Mn concentration,the activity of superoxide dismutase(SOD)increased first,then decreased and then increased,reaching a peak in Mn1 treatment,which was significantly higher than that of Mn0 by 8%,and Mn3 treatment was significantly lower than that of Mn0 by 21%.Compared with Mn0,the peroxidase(POD)activity of Mn2,Mn3 and Mn4 significantly decreased.With the increase of Mn concentration,the content of indole-3-acetic acid(IAA)decreased first,then increased and then decreased,and Mn3 and Mn4 were significantly lower than Mn0 by 20%−25%.The gibberellin(GA)content increased first and then decreased with the increase of Mn concentration,reaching a peak in Mn2 treatment,which was 8%higher than that of Mn0.With the increase of Mn concentration,the content of abscisic acid(ABA)decreased and increased,and Mn4 was significantly increased by 13%compared with Mn0,and Mn2 was significantly decreased by 6%compared with Mn0.2)Field experiments Root biomass increase

关 键 词：黄芩 氯化锰 生长发育 黄酮类化合物 激素 抗氧化酶 叶面喷施 

分 类 号：R28[医药卫生—中药学]