参芪降糖胶囊通过调节NF-κB/NLRP3通路对糖尿病动脉粥样硬化大鼠糖脂代谢、内皮细胞功能的影响  

Impacts of Shenqi Jiangtang Capsules on glucose and lipid metabolism and endothelial cell function in diabetes atherosclerotic rats by regulating the NF-κB/NLRP3 pathway

作　　者：张金凤 周聪 ZHANG Jinfeng;ZHOU Cong(Chongzhou Hospital of Traditional Chinese Medicine,Chengdu 611230,China)

机构地区：[1]崇州市中医医院,成都611230

出　　处：《天津中医药大学学报》2025年第3期241-248,共8页Journal of Tianjin University of Traditional Chinese Medicine

摘　　要：[目的]探讨参芪降糖胶囊(简称SJC)对糖尿病动脉粥样硬化(AS)大鼠糖脂代谢、内皮细胞功能的影响及对核因子(NF)-κB/NOD样受体蛋白3(NLRP3)信号通路的调节作用。[方法]建立AS大鼠模型,所有大鼠随机分为空白组、模型组、SJC低剂量组、SJC高剂量组和SJC高剂量+NLRP3激活剂组,观察各组大鼠一般情况,试剂盒检测血清空腹血糖(FBG)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、白介素(IL)-1β、IL-6、肿瘤坏死因子(TNF)-α含量,自动分析仪检测血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)的含量,苏木精-伊红(HE)染色观察主动脉病理学特征;敏感肌张力描计检测动脉血管内皮功能;免疫蛋白印迹(Western blot)技术验证磷酸化(p)-NF-κB p65、NF-κB p65、NLRP3蛋白表达;免疫组化检测主动脉半胱天冬酶-1(Cleaved-Caspase-1)、凋亡相关斑点样蛋白(ASC)蛋白表达。[结果]与空白组对比,模型组大鼠体型瘦弱,毛发干枯,多食多饮多尿,精神恍惚,主动脉管腔内膜不规则增厚且形成斑块,炎症细胞浸润,平滑肌排列不规则,血清FBG、FINS、HOMA-IR、TC、TG、LDL-C、IL-1β、IL-6、TNF-α及主动脉内膜p-NF-κB p65/NF-κB p65、NLRP3、Cleaved-Caspase-1、ASC蛋白表达水平升高,血管内皮最大舒张率和HDL-C水平下降(P<0.05);与模型组比较,SJC低、高剂量组大鼠毛色光泽增加,精神状态和多饮多尿状况好转,血管内斑块减少,中膜钙化少见,炎性细胞浸润减少,整体病变程度减轻,血清FBG、FINS、HOMA-IR、TC、TG、LDL-C、IL-1β、IL-6、TNF-α及主动脉内膜p-NF-κB p65/NF-κB p65、NLRP3、Cleaved-Caspase-1、ASC蛋白表达水平降低,血管内皮最大舒张率和HDL-C水平升高(P<0.05);与SJC高剂量组对比,SJC高剂量+NLRP3激活剂组大鼠DM体征和血管病变程度加重,血清FBG、FINS、HOMA-IR、TC、TG、LDL-C、IL-1β、IL-6、TNF-α及主动脉内膜p-NF-κB p65/NF[Objective]To investigate the impacts of Shengi Jiangtang Capsules(SJC)on glucose and lipid metabolism,endothelial cell function in diabetes atherosclerosis(AS)rats,and its regulatory effect on the nuclear factor(NF)-κB/NOD like receptor protein 3(NLRP3)signaling pathway.[Methods]An AS rat model was established,and all rats were randomly grouped into blank group,model group,SJC low-dose group,SJC high-dose group,and SJC high-dose+NLRP3 activator group,the general situation of rats in each group was observed,the kit was applied to detect the levels of serum fasting blood glucose(FBG),fasting insulin(FINS),insulin resistance index(HOMA-IR),interleukin-1β(IL-1β),IL-6,and tumor necrosis factor-α(TNF-α),the automatic analyzer was applied to detect the contents of serum total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C),HE staining was applied to observe the pathological characteristics of the aorta;the sensitive tonometer was applied to detect the endothelial function of arteries;Western blot was applied to verify the protein expression of phosphorylated(p)-NF-κB p65,NF-κB p65,NLRP3;the expressions of Cleaved-Caspase-1(Cleaved-Caspase-1)and apoptosis-related speck-like protein(ASC)in aorta were detected by immuno-histochemistry.[Results]Compared with the blank group,the rats in the model group had a lean and weak body shape,dry hair,excessive eating,drinking and urination,confusion,irregular thickening and formation of plaques in the aortic lumen,infiltration of inflammatory cells,and irregular arrangement of smooth muscle,the levels of serum FBG,FINS,HOMA-IR,TC,TG,LDL-C,IL-1β,IL-6,TNF-α,and the protein expression levels of p-NF-κB p65/NF-κB p65,NLRP3,Cleaved-Caspase-1,and ASC in the aortic intima increased,the maximum diastolic rate of vascular endothelium and HDL-C level decreased(P<0.05);compared with the model group,the fur color and luster of the rats in the low-dose and high-dose SJC groups were improved,their mental state a

关 键 词：参芪降糖胶囊 糖尿病动脉粥样硬化 核因子-κB/NOD样受体蛋白3通路 糖脂代谢 内皮细胞 

分 类 号：R587.1[医药卫生—内分泌]