Single-Round Circular Aptamer Discovery Using Bioinspired Magnetosome-Like Magnetic Chain Cross-Linked Graphene Oxide  

作　　者：Lili Yao Junmei Feng Yuefei Zhou Shengjie Gao Shuai Liu Hao Qu Yu Mao Lei Zheng 

机构地区：[1]School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China

出　　处：《Research》2025年第1期23-34,共12页研究(英文)

基　　金：supported by the National Natural Science Foundation of China(22104028,32072306,and U23A20265).

摘　　要：Circular aptamers are promising candidates for analytical and therapeutic applications due to their enhanced biological and structural stability.However,the process of circular aptamer selection remains a great challenge,as it requires multiple rounds of binding-separation-amplification that involves issues with nonspecific binding and amplification bias.Here,we develop a highly practical solution for reliable selection of circular aptamers in a single round based on magnetosome-like magnetic chain cross-linked graphene oxide(separation efficiency≈105).High-affinity aptamer candidates can be rapidly selected from a preenriched circular DNA library,while low-affinity candidates are effectively adsorbed and separated by magnetosome-like magnetic chain cross-linked graphene oxide.With lipopolysaccharide as a representative model,the single-round selected lipopolysaccharide circular aptamer has been identified to have a high binding affinity with a Kd value of low to nanomolar range.Using this method,circular aptamers for protein and small-molecule targets were also successfully generated.We envision that this approach will accelerate the discovery of various new circular aptamers and open up a new avenue for analytical and therapeutic studies.

关 键 词：lipopolysaccharide analytical therapeutic applications magnetosome magnetic chain graphene oxide high affinity aptamer selection protein targets single round circular aptamer discovery circular aptamers 

分 类 号：O657.3[理学—分析化学]