Spatiotemporal Observation of Monosodium Urate Crystals Deposition in Synovial Organoids Using Label-Free Stimulated Raman Scattering  

作　　者：Yaxin Chen Ziyi Chen Wenjuan Wang Yinghui Hua Minbiao Ji 

机构地区：[1]State Key Laboratory of Surface Physics and Department of Physics,Human Phenome Institute,Academy for Engineering and Technology,Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education),Shanghai Key Laboratory of Metasurfaces for Light Manipulation,Fudan University,Shanghai 200433,China [2]Department of Sports Medicine,Huashan Hospital,Fudan University,Shanghai,China

出　　处：《Research》2025年第1期246-256,共11页研究(英文)

基　　金：supported by the National Key R&D Program of China(grant numbers 2021YFF0502900 and SQ 2020YFA0803800);the National Natural Science Foundation of China(grant number 82072510);the Shanghai Science and Technology Committee(grant numbers 22dz1204702 and 23dz2260100)。

摘　　要：Gout,a common form of arthritis,is characterized by the deposition of monosodium urate(MSU)crystals in joints.MSU deposition in synovial tissues would initiate arthritis flares and recurrence,causing irreversible joint damage.However,the dynamic deposition of MSU crystals in tissues lacks experimental observation.In this study,we used chemical-specific,label-free stimulated Raman scattering(SRS)microscopy to investigate the spatiotemporal deposition and morphological characteristics of MSU crystals in human synovial organoids.Our findings revealed a critical 12-h window for MSU deposition in the lining layer of gouty synovium.Moreover,distinctive inflammatory reactions of the lining and sublining synovial layers in gout using SRS microscopy were further verified by immunofluorescence.Importantly,we identified a crucial proinflammatory role of sublining fibroblast-like synoviocytes,indicating a need for targeted medication treatment on these cells.Our work contributes to the fundamental understanding of MSU-based diseases and offers valuable insights for the future development of targeted gout therapies.

关 键 词：Monosodium urate crystals arthritis flares Stimulated Raman scattering microscopy Spatiotemporal observation Synovial organoids Fibroblast synoviocytes synovial tissues Inflammatory reactions 

分 类 号：R684.3[医药卫生—骨科学]