抗生素对抗性/非抗性大肠杆菌的选择性作用  

Selective Action of Antibiotics on Resistant/Non-Resistant Escherichia Coli

作　　者：谢依侨 陈智 于洋[1] XIE Yiqiao;CHEN Zhi;YU Yang(Guangdong Provincial Environmental Science and Engineering Experimental Teaching Demonstration Center,Guangdong Provincial Key Laboratory of Environmental Pollution and Health,College of Environment and Climate,Jinan University,Guangzhou Guangdong 511436,China;School of Basic Medicine and Public Health,Jinan University,Guangzhou Guangdong 511443,China)

机构地区：[1]暨南大学环境与气候学院,广东省环境污染与健康重点实验室,广东省环境科学与工程实验教学示范中心,广东广州511436 [2]暨南大学基础医学与公共卫生学院,广东广州511443

出　　处：《当代化工》2025年第3期517-522,588,共7页Contemporary Chemical Industry

基　　金：国家自然科学基金面上项目(项目编号:42277212);广东省基础与应用基础研究基金青年提升项目(项目编号:2023A1515030281);暨南大学2023年“四新”实验教学课程改革项目(项目编号:SYJG202322、SYJG202326)。

摘　　要：在深刻认识抗生素的滥用和过度排放已经衍生出大量抗性菌和抗性基因的背景下,验证抗生素的存在会使得抗性菌成为优势菌,并深入理解抗生素选择性作用对抗性菌丰度的影响。实验室配制抗性/非抗性大肠杆菌混合溶液,模拟实际水样,通过药敏试纸法和酶标仪法测定阿莫西林投加下抗性/非抗性大肠杆菌丰度变化,酶标仪法测定阿莫西林溶液中游离质量浓度与总质量浓度(溶液中与细胞吸附质量浓度之和),证明抗性/非抗性大肠杆菌均通过吸附作用去除溶液中的抗生素,一旦抗性菌死亡,细胞破裂后,抗生素又会重新释放到环境中,抗性细菌丰度增加并不能从根本上解决抗生素污染问题。In the context of a deep understanding that the abuse and excessive discharge of antibiotics have spawned a large number of resistant bacteria and resistance genes,it is necessary to verify the presence of antibiotics will make resistant bacteria become dominant bacteria and to deeply understand the impact of antibiotic selective effects on the abundance of resistant bacteria.In this paper,a mixed solution of resistant/non-resistant E.coli was prepared to simulate the actual water sample.The changes in the abundance of resistant/non-resistant E.coli under the addition of amoxicillin were determined by the drug sensitivity test paper method and the enzyme marker method.The free mass concentration and total mass concentration(the sum of mass concentration in the solution and the cell adsorption mass concentration)of amoxicillin in the solution were determined by the enzyme marker method.It was proved that both resistant and non-resistant E.coli removed antibiotics from the solution through adsorption.Once the resistant bacteria died and the cells ruptured,the antibiotics were released back into the environment.The increase in the abundance of resistant bacteria did not fundamentally solve the problem of antibiotic pollution.

关 键 词：抗性大肠杆菌 阿莫西林 抗生素选择性作用 生物技术 废水 吸附作用 修复 

分 类 号：TQ085.413[化学工程]