不同剂量四君子汤颗粒对多囊卵巢综合征模型大鼠炎症状态和脂代谢的影响  

Effects of different doses of Si Jun zi Decoction granules on the inflammatory status and lipid metabolism in the model ratwith polycystic ovary syndrome

作　　者：何珊珊 马思琪 赵锡曼 郜艳翠 孙婷 曲银娥 HE Shanshan;MA Siqi;ZHAO Ximan;GAO Yancui;SUN Ting;QU Yin'e(School of Basic Medicine,North China University of Science and Technology,Tangshan O63210;Reproductive Genetics Department,Tangshan Maternal and Child Health Hospital,Tangshan 063014;Key Laboratory of Basic Medical Sciences for Chronic Diseases,Hebei Province,Tangshan 063210,China)

机构地区：[1]华北理工大学基础医学院,河北省唐山市063210 [2]唐山市妇幼保健院生殖遗传科,河北省唐山市063014 [3]河北省慢性疾病基础医学重点实验室,河北省唐山市063210

出　　处：《中国煤炭工业医学杂志》2025年第1期18-30,共13页Chinese Journal of Coal Industry Medicine

基　　金：河北省引进留学人员项目(编号:No.201860)。

摘　　要：目的探讨不同剂量四君子汤颗粒对多囊卵巢综合征大鼠(PCOS)炎症状态和脂代谢的影响。方法选取30只3周龄雌性SD大鼠,随机分为对照组(CON组,n=6)和PCOS造模组(n=24),造模组采用来曲唑(1mg/kg·d,溶于0.5%羧甲基纤维素钠溶液)灌胃联合高脂饲料喂养,CON组采用等量0.5%羧甲基纤维素钠溶液灌胃,普通饲料喂养,连续28d。造模成功后将造模组分为PCOS组、四君子汤低剂量组(L-SJZD组)、中剂量组(M-SJZD组)和高剂量组(H-SJZD组),每组6只,四君子汤组分别灌胃5.25g/kg·d、10.5g/kgd、21g/kg·d四君子汤颗粒,PCOS组与CON组灌胃等量生理盐水,均以普通饲料喂养,连续21d。记录大鼠动情周期,称重大鼠体质量及卵巢并计算卵巢指数,HE染色法观察卵巢组织形态学变化,酶联免疫吸附试验(ELISA)测定大鼠血清睾酮(T)、黄体生成素(LH)、卵泡刺激素(FSH)水平,Westernblot法检测卵巢组织中白介素-1β(IL-1β)、白介素-18(IL-18)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、C反应蛋白(CRP)、白介素-4(IL-4)蛋白,全自动生化分析仪测定大鼠血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)水平。结果与CON组比较,PCOS造模组大鼠动情周期紊乱,卵巢呈多囊样改变,卵巢指数升高(F=20.700,P<0.05),血清T、LH升高(t=22.740,t=43.040,P<0.05),FSH降低(t=9.264,P<0.05),提示造模成功。经四君子汤颗粒治疗后,大鼠动情周期恢复,卵巢多囊样变化改善;与PCOS组比较,各治疗组大鼠血清T、LH、LH/FSH均降低(F=231.100,F=483.100F=200.900,P<0.05),FSH均升高(F=66.930,P<0.05),M-SJZD组及H-SJZD组T、LH、LH/FSH均低于L-SJZD组(P<0.05),FSH均高于L-SJZD组(P<0.05)。与CON组比较,PCOS组大鼠卵巢组织促炎因子IL-1β、IL-18、IL-6、TNF-α和CRP蛋白表达水平上调(F=116.000,F=46.960,F=166.200,F=185.500,F=66.150,P<0.05),而抗炎因子IL-4蛋白表达下调(F=154.100,P<0.05);与PCOS组比较,各治疗组大鼠卵巢组织Objective To investigate the ffects of diferent doses of Si Jun Zi Decoction granules on the inflammatory status and lipid metabolism in rats with polycystic ovary syndrome(PCOS).Methods Thirty 3-week-old female SD rats were randomly divided into a control group(CON group,n=6)and a PCOS modeling group(n=24).The PCOS modeling group received gavage of letrozole(1 mg/kg d,dissolved in 0.5%carboxymethyl cellulose sodium solution)combined with a highfat diet for 28 consecutive days,while the CON group was administered an equivalent volume of 0.5%carboxymethyl cellulose sodium solution and regular diet.Following successful modeling,the PCOS group was further divided into a PCOS group,a low-dose Si Jun Zi Decoction group(L-SJZD group),a medium-dose group(M-SJZD group),and a high-dose group(H-SJZD group),with 6 rats in each group.The Si Jun Zi Decoction groups received gavage of 5.25 g/kg d,10.5 g/kg d,and 21 g/kg·d of Si Jun Zi Decoction granules,while the PCOS and CON groups were administered an equivalent volume of saline,all maintained on a regular diet for 21 days.Estrous cycles were recorded,ovarian morphological changes were observed through HE staining,the ovarian index was measured and recorded,and serum levels of testosterone(T),luteinizing hormone(LH),and follicle-stimulating hormone(FSH)were measured using ELISA.Western blot analysis was employed to detect the protein expressions of interleukin-1β(IL-1β),interleukin-18(IL-18),interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α),C-reactive protein(CRP),and interleukin-4(IL-4)in ovarian tissues.A fully automatic biochemical analyzer was used to measure serum cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)levels.Results The PCOS modeling group exhibited disrupted estrous cycles,and HE staining revealed polycystic changes in the ovaries.Compared to the CON group,the ovarian index of PCOS group was increased(F=20.700,P<0.05),serum levels of T and LH were elevated(t=22.740,t=43.040,P

关 键 词：多囊卵巢综合征 模型大鼠 四君子汤 炎症 脂质代谢 

分 类 号：R711.75[医药卫生—妇产科学]