3,4-二硝基呋咱基氧化呋咱皮肤致敏研究  

Study of skin sensitization of 3,4-Bis(3-nitrofurazan-4-yl)furoxan

作　　者：赵彬 李欢 刘志永 牛浩 赵阳阳 高俊宏 ZHAO Bin;LI Huan;LIU Zhi-yong;NIU Hao;ZHAO Yang-yang;GAO Jun-hong(Institute for Hygiene of Ordnance Industry,Xi'an,Shaanxi 710065,China;Xi′an Key Laboratory of Toxicology and Biological Effects,Xi'an,Shaanxi 710065,China)

机构地区：[1]兵器工业卫生研究所毒理技术研究中心,陕西西安710065 [2]西安市毒理与生物效应重点实验室,陕西西安710065

出　　处：《毒理学杂志》2025年第1期40-44,共5页Journal of Toxicology

基　　金：陕西省创新能力支撑计划,DNTF变应性接触性皮炎动物模型构建及发病机制研究(2024CX-GXPT-47)。

摘　　要：目的通过豚鼠局部封闭涂皮实验和小鼠局部淋巴结法研究3,4-二硝基呋咱基氧化呋咱[3,4-bis(3-nitrofurazan-4-yl)furoxan,DNTF]的皮肤致敏性。方法以2,4-二硝基氯苯为阳性对照,采用豚鼠局部封闭涂皮实验(BT法)和局部淋巴结法(local lymph node assay,LLNA),对DNTF进行致敏性研究。结果豚鼠染毒24和48 h后致敏率分别为35%和50%,中度致敏性;LLNA:DA法检测显示刺激指数(stimulus indexes,SI)>1.8,中度致敏物;LLNA:BrdU-ELISA法检测显示SI>1.6,中度致敏物。结论DNTF具有致敏性,为中等致敏物。为保护接触者的身体健康提供基础数据,同时为小鼠局部淋巴结法在国内化学品毒性评价中的发展和应用积累了实验数据。Objective To detect skin sensitization of 3,4-Bis(3-nitrofurazan-4-yl)furoxan(DNTF)by a local lymph node assay in mice and the occluded patch test of Buchler(BT).Methods According to BT test and local lymph node assay(LLNA)standard method,2,4-dinitrochlorobenzene was used as the positive control to detect sensitization of DNTF.Results The result from the BT test show that DNTF was a moderate sensitizer.The result from LLNA test show that the stimulus indexes of 10%DNTF were SI>1.8,and SI>1.6,and DNTF was a moderate sensitizer.Conclusion DNTF can cause skin sensitization,it is a moderate sensitizer.This study might provide basic data for protecting the health of contacts,and accumulate experimental data for the development and application of local lymph node method in chemical toxicity evaluation in China.

关 键 词：3 4-二硝基呋咱基氧化呋咱 皮肤致敏 豚鼠局部封闭涂皮实验 小鼠局部淋巴结法 

分 类 号：R114[医药卫生—卫生毒理学] R99[医药卫生—公共卫生与预防医学]