连翘苷抗突变作用实验研究  

Anti-mutagenic activity of phillyrin:an experimental evaluation

作　　者：李学敏[1] 李淑琴[1] 边林秀[1] 张颖 王晓瑞[1] 王利如 LI Xue-min;LI Shu-qin;BIAN Lin-xiu;ZHANG Ying;WANG Xiao-rui;WANG Li-ru(Center for Disease Control and Prevention in Shanxi Province,Toxicology Department,Shanxi 030012,China;School of Public Health,Shanxi Medical University,Shanxi 030001,China)

机构地区：[1]山西省疾病预防控制中心,山西太原030012 [2]山西医科大学公共卫生学院,山西太原030001

出　　处：《毒理学杂志》2025年第1期60-62,67,共4页Journal of Toxicology

基　　金：山西省疾病预防控制中心第二批“五个一流”科研项目(05)。

摘　　要：目的通过细菌回复突变试验和小鼠骨髓细胞微核试验,探究连翘苷对TA98、TA100细菌突变株以及小鼠骨髓细胞的抗突变作用。方法采用细菌回复突变试验、骨髓细胞微核试验方法,选用不同浓度的连翘苷进行了抗突变试验及结果分析。结果连翘苷高剂量组(5 mg/皿)对阳性物所诱导的细菌回复突变菌株TA98和TA100的回复突变菌落数均有抑制作用(P<0.05),45 mg/kg剂量组对环磷酰胺诱导的小鼠骨细胞微核发生率有明显的抑制作用(P<0.05)。结论连翘苷具有一定的抗突变作用。Objective The anti-mutagenic effect of phillyrin on TA98 and TA100 bacterial mutants and mouse bone marrow cells was evaluated by the bacterial revertant mutation assay and the bone marrow micronucleus test.Methods In this study,the bacterial reverse mutation assay and the bone marrow micronucleus test were used to analyze the anti-mutagenic effect of different concentrations of phillyrin.Results Compared with the positive control-induced bacterial revertant mutant strains TA98 and TA100,the high-dose phillyrin(5 mg/plate)decreased the number of revertant mutant colonies significantly(P<0.05).Additionally,phillyrin 45 mg/kg showed a notable inhibitory effect on the incidence of micronuclei which was induced by cyclophosphamide in mouse bone marrow cells(P<0.05).Conclusion The result demonstrated that phillyrin exhibited anti-mutagenic properties.

关 键 词：连翘苷 抗突变作用 细菌回复突变 微核 

分 类 号：R114[医药卫生—卫生毒理学] R99[医药卫生—公共卫生与预防医学]