2021-2022年中国5岁以下急性胃肠炎相关腺病毒分子特征分析  

Molecular characteristics of human adenovirus related to acute gastroenteritis among children under 5 years old in China,2021-2022

作　　者：董梦洁 车如意 熊光萍 范佳欣 唐晓苹 张无敌 李响 周帅锋 康倩 傅忠燕 曹冉冉 唐梅荣 李伟红 王宇 雷玥 李静 李楚楚 王倩 袁媛 段招军[1] 李丹地[1] Dong Mengjie;Che Ruyi;Xiong Guangping;Fan Jiaxin;Tang Xiaoping;Zhang Wudi;Li Xiang;Zhou Shuaifeng;Kang Qian;Fu Zhongyan;Cao Ranran;Tang Meirong;Li Weihong;Wang Yu;Lei Yue;Li Jing;Li Chuchu;Wang Qian;Yuan Yuan;Duan Zhaojun;Li Dandi(National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,NHC Key Laboratory of Medical Virology and Viral Diseases,Beijing 102206,China;National Viral Diarrhea Surveillance Network Laboratory,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所、传染病溯源预警与智能决策全国重点实验室、国家卫生健康委员会医学病毒和病毒病重点实验室,北京102206 [2]全国病毒性腹泻监测网络实验室,北京102206

出　　处：《国际病毒学杂志》2025年第1期1-6,共6页International Journal of Virology

基　　金：国家重点研发计划(2022YFC2304302);国家自然科学基金(21934005)。

摘　　要：目的了解我国5岁以下急性胃肠炎患儿中人腺病毒(human adenovirus,HAdV)的分子特征和遗传进化规律,为疾病控制和预防提供基础数据。方法收集2021—2022年中国14省5岁以下因急性胃肠炎住院或门诊患儿粪便样本1321份,对样本进行HAdV实时荧光检测,对HAdV阳性样本和进行测序,利用BioAider 1.532、MEGA 11.0和BEAST 1.8软件对HAdV样本进行同源性、基因分型、系统发育分析。结果1321份样本中HAdV阳性74例(5.60%),包括4种HAdV亚型,HAdV-F占比高达89.19%,其次为HAdV-C(5.41%)、HAdV-A(4.05%)和HAdV-B(1.35%)。对占比最高的HAdV-F41基因型进行贝叶斯进化分析,HAdV-F41最近共同祖先时间约为1882年,平均进化率约为3.69×10^(-5)(HPD95%5.01×10^(-6)~8.41×10^(-5))替换/位点/年。结论我国5岁以下急性胃肠炎患儿中HAdV型别多样,HAdV-F41是最优势基因型,生物信息学分析结果显示我国HAdV进化速率较慢,且主要流行谱系暂未受到欧洲地区影响。对HAdV进行系统长期的监测有助于了解其在中国的多样性及其进化规律。Objective To understand the molecular characteristics and genetic evolution of human adenovirus(HAdV)in children under 5 years of age with acute gastroenteritis in China,so as to provide basic data for disease control and prevention.MethodsFrom 2021 to 2022,1321 faecal samples were collected from children under five years of age who were hospitalized or visiting outpatient for acute gastroenteritis in 14 provinces of China.The samples were tested for HAdV using real time fluorescence PCR and the HAdV positive samples were sequenced.Homology,genotyping and phylogenetic analyses of HAdV samples were conducted using the softwares BioAider 1.532,MEGA 11.0 and BEAST 1.8.ResultsFor the 1321 fecal samples,74(5.60%)were positive for HAdV.There were 4 genotypes of HAdV and HAdV-F accounted as high as 89.19%,followed by HAdV-C(5.41%),HAdV-A(4.05%)and HAdV-B(1.35%).The Bayesian evolutionary analysis was conducted for the HAdV-F41 which was the dominant genotype,and the result demonstrated that the Most Recent Common Ancestor(TMRCA)of HAdV-F41 was around 1882 and the average evolutionary rate.The average evolutionary rate was approximately 3.69×10^(-5)substitutions per site per year(HPD95%5.01×10^(-6)to 8.41×10^(-5)).ConclusionsThere were different HAdV genotypes among children under 5 years old with acute gastroenteritis in China and HAdV-F41 was the dominant genotype.Bioinformatics analyses indicated that the evolutionary rate of HAdV in China was relatively slow and the main epidemiological lineage were not affected significantly by the European region.Long-term surveillance of HAdV would assist understanding the diversity and evolutionary pattern of the virus in China.

关 键 词：人腺病毒 基因型 急性胃肠炎 分子特征 

分 类 号：R37[医药卫生—病原生物学]