一株Wa-like中国G3P[8]-I5-A8基因型轮状病毒重配株全基因组特征分析  

Whole genome characterization of a Wa-like Chinese rotavirus reassortant strain of the G3P[8]-I5-A8 genotype

作　　者：唐晓苹 熊光萍 车如意 董梦洁 刘颂 张无敌 王宏[2] 章青[2] 孔翔羽[2] 庞立丽[2] 李丹地[2] Tang Xiaoping;Xiong Guangping;Che Ruyi;Dong Mengjie;Liu Song;Zhang Wudi;Wang Hong;Zhang Qing;Kong Xiangyu;Pang Lili;Li Dandi(School of Public Health,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,NHC Key Laboratory of Medical Virology and Viral Diseases,Beijing 102206,China;Viral Disease Laboratory,Qingdao Center for Disease Control and Prevention,Qingdao 266000,China)

机构地区：[1]甘肃中医药大学公共卫生学院,兰州730000 [2]中国疾病预防控制中心病毒病预防控制所、传染病溯源预警与智能决策全国重点实验室,国家卫生健康委员会医学病毒和病毒病重点实验室,北京102206 [3]青岛市疾病预防控制中心病毒性疾病检验所,266000

出　　处：《国际病毒学杂志》2025年第1期12-16,共5页International Journal of Virology

基　　金：国家重点研发计划(2022YFC2304302,2018YFC1200602);国家自然科学基金(21934005)。

摘　　要：目的研究一株Wa-like中国G3P[8]-I5-A8基因型RVA重配株全基因组特征,并分析与RVA疫苗株的抗原表位之间差异。方法采用反转录-聚合酶链式反应(RT-PCR)方法,对2023年中国青岛采集的一份G3P[8]基因型RVA标本进行全序列扩增。利用在线RVA自动分型工具(http://rotac.regatools.be)对测序结果进行分型,应用MEGA 11.0、Geneious Prime 2023.2.1、Geneious 9.0.2和DNASTAR 5.1等软件对RVA毒株进行全序列遗传变异和进化分析。结果成功获取一株G3P[8]基因型RVA(QD01)的全基因组序列,该毒株具有独特的类似Wa-like的基因型:G3-P[8]-I5-R1-C1-M1-A8-N1-T1-E1-H1。与RVA疫苗株RotaTeq^(TM)(G3)、LLR和Rotarix的VP7中和抗原表位对比有4~13个差异且均在抗原表位的关键位置7-1 b差异较大。结论本研究RVA毒株QD01为G3-P[8]-I5-A8基因型,遗传进化分析推测该毒株可能是亚洲地区猪源RVA和人源RVA毒株间发生重配产生的新型毒株,QD01与RVA疫苗株在抗原表位的关键位置差异较大。Objective To study the genome-wide characteristics of a Wa-like Chinese G3P[8]-I5-A8 genotype rotavirus(RVA)reassortant strain and analyze the differences in antigenic epitopes between the and RVA vaccine strain.MethodsReverse transcription-polymerase chain reaction(RT-PCR)was used to amplify the complete genome sequence of a RVA G3P[8]genotype strain from the specimen collected in Qingdao,China,in 2023.The sequencing result were typed using the online RVA automatic typing tool(http://rotac.regatools.be).The software,including MEGA 11.0,Geneious Prime 2023.2.1,Geneious 9.0.2 and DNASTAR 5.1 were applied to analyze the whole genome sequence for the genetic variation and evolution.ResultsThe whole genome sequence of a G3P[8]genotype RVA strain(QD01)was obtained.The strain had a unique Wa-like genotype,G3-P[8]-I5-R1-C1-M1-A8-N1-T1-E1-H1.There were 4-13 differences in VP7 epitopes between QD01 and RVA vaccine strains RotaTeq^(TM)(G3),LLR and Rotarix.All differences showed significant variation at the key position 7-1b of the antigenic epitope.ConclusionsIn this study,the RVA strain QD01 belonged to G3-P[8]-I5-A8 genotype.Genetic evolutionary analysis hypothesized that this strain might be a novel strain generated by reassortment between porcine-and human-origin RVA strains from Asia,and QD01 had significant difference at the key positions of antigenic epitopes comparing to vaccine strains.

关 键 词：轮状病毒 基因型 分子特征 全基因组 抗原位点 

分 类 号：R54[医药卫生—心血管疾病]