植物还原法制备金纳米粒子用于检测汞离子  

Gold nanoparticles prepared by plant reduction for visual mercury ions detection

作　　者：韩桂美 张扬 HAN Guimei;ZHANG Yang(College of Chemistry and Chemical Engineering,Qilu Normal University,Jinan 250200,China)

机构地区：[1]齐鲁师范学院化学与化工学院,济南250200

出　　处：《分析试验室》2025年第3期353-358,共6页Chinese Journal of Analysis Laboratory

基　　金：齐鲁师范学院博士基金项目(BS2017023)资助。

摘　　要：本研究选择菠菜提取液为生物还原剂,通过植物还原法制备了金纳米粒子(Au NPs)并将其用于检测汞离子(Hg^(2+))。所合成的Au NPs均一、稳定,粒径约为40 nm。在检测体系中Hg^(2+)被还原成Hg,生成的Hg附着在Au NPs的表面生成金汞齐(Au@Hg),表现出较强的过氧化物酶活性。基于Hg^(2+)对Au NPs的过氧化物酶增强作用,Au@Hg催化过氧化氢(H_(2)O_(2))产生羟基自由基并将无色的四甲基联苯胺(TMB)氧化为蓝色的氧化四甲基联苯胺(ox TMB),据此建立了检测Hg^(2+)的方法。加入Hg^(2+)前后样品在652 nm处的吸光度值差值(A-A_(0))与加入的Hg^(2+)的浓度(c)在1.56~10μmol/L范围内呈线性关系,线性方程为A-A_(0)=0.0818c-0.00732,相关系数(R^(2))为0.9948,检测限(LOD)为0.41μmol/L。所建立的方法有望用于Hg^(2+)的检测。Selecting spinach extract as the biological reductant,gold nanoparticles (Au NPs) were prepared by plant reduction method and applied to the detection of mercury ions (Hg^(2+)).The synthesized Au NPs were uniform and stable,with a particle size of~40 nm.In the system,Hg^(2+)was reduced to Hg,which attached to the surface of the Au NPs to generate Au@Hg showing strong peroxidase activity.Based on the peroxidase-enhancing effect of Hg^(2+)on the Au NPs,Au@Hg catalyzed hydrogen peroxide to generate hydroxyl radicals which can oxidize colorless tetramethylbenzidine (TMB) to blue oxidized tetramethylbenzidine (ox TMB),thus a method for detection of Hg^(2+)was established.There was linear relationship between the absorbance difference (A-A_(0)) at 652 nm before and after the addition of Hg^(2+)and the concentration of Hg^(2+)(c) in the range of 1.56-10μmol/L,with the linear regression equation of A-A_(0)=0.0818c?0.00732,and the correlation coefficient (R~2) of 0.9948.The detection limit (LOD) was 0.41μmol/L.The method is expected to be applied for the detection of Hg^(2+).

关 键 词：菠菜提取液 金纳米粒子 金汞齐 过氧化物酶 汞离子(Hg^(2+)) 

分 类 号：O657.31[理学—分析化学] X832[理学—化学]