基于量子点微球免疫层析法快速灵敏检测畜禽中的喹乙醇  

Quantum dot nanobeads-based lateral flow assay for rapid and sensitive detection of olaquindox in livestock

作　　者：费丹 徐俊[1,2] 广业兰 李凡 谢敏 周瑶敏[1] 陈媛 胡利珍[2] 封帆 罗凯 FEI Dan;XU Jun;GUANG Yelan;LI Fan;XIE Min;ZHOU Yaomin;CHEN Yuan;HU Lizhen;FENG Fan;LUO Kai(Key Laboratory for Quality and Safety Control of Poultry Products,Ministry of Agriculture and Rural Affairs of the People's Republic of China,Institute for Quality&Safety and Standards of Agricultural Products Research,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;Institute of Animal Husbandry and Veterinary Science,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;Jiangxi Weibang Biotechnology Co.,Ltd.,Nanchang 330029,China)

机构地区：[1]农业农村部禽类产品质量安全控制重点实验室,江西省农业科学院农产品质量安全与标准研究所,南昌330200 [2]江西省农业科学院畜牧兽医研究所,南昌330200 [3]江西维邦生物科技有限公司,南昌330029

出　　处：《分析试验室》2025年第3期418-423,共6页Chinese Journal of Analysis Laboratory

基　　金：江西省重点研发计划(20201BBF61008);江西省肉牛创新联盟项目(JXXTCX201702)资助。

摘　　要：选择具有高发光强度的量子点荧光微球作为信号标记物,建立了一种快速灵敏检测喹乙醇的量子点微球免疫层析法。对探针偶联pH、碳二亚胺盐酸盐(EDC)用量、喹乙醇抗体浓度、 T线抗原浓度和探针体积进行了优化。通过量子点微球试纸条检测仪读取试纸条上检测线和质控线的荧光信号强度,以喹乙醇浓度为横坐标,以检测线(T)和质控线(C)荧光信号强度的比值(T/C)为纵坐标,建立标准曲线。结果表明,该方法定量检测范围为0.25~5μg/L,半抑制率为1.24μg/L,回收率在83.6%~118.9%之间,与卡巴氧、喹胺醇和喹烯酮等结构类似物的交叉反应率均小于0.1%。该方法适用于大批量样品的现场快速筛查。Quantum dot fluorescent nanobeads with high luminescence intensity were selected as signal markers to establish a fast and sensitive quantum dot nanobeads immunochromatography method for detecting olaquindox.The pH,carbodiimide hydrochloride(EDC) dosage,concentrations of olaquindox and T-line antigen,and probe volume were optimized.With a quantum dot strip reader,the fluorescent signal intensity of test line(T) and control line(C) on lateral flow strip was recorded.The standard curve was established by plotting the T/C ratio against the concentration of olaquindox.The results indicated that the quantitative detection range of this method was 0.25-5 μg/L,and the half inhibition rate is 1.24 μg/L.The recovery rates ranged from 83.6% to 118.9%,and the cross-reactivity with structurally similar compounds such as carbadox,kuianchun,and quinocetone were all less than 0.1%.This method is suitable for rapid on-site screening of large quantities of samples.

关 键 词：量子点微球 免疫层析法 喹乙醇 

分 类 号：O657[理学—分析化学]