电化学多层组装纳米金和电还原氧化石墨烯修饰电极的非酶葡萄糖传感  

Electrochemical multilayer assembly of gold and electro-reduced graphene oxide modified electrodes for non-enzymatic glucose sensing

作　　者：杨妍[1] 王晴 张雯钰 张叶臻[1] 关香叶 闫鑫桀 YANG Yan;WANG Qing;ZHANG Wenyu;ZHANG Yezhen;GUAN Xiangye;YAN Xinjie(College of Chemistry and Pharmacy Engineering,Nanyang Normal University,Nanyang 473061,China)

机构地区：[1]南阳师范学院化学与制药工程学院,南阳473061

出　　处：《分析试验室》2025年第3期424-431,共8页Chinese Journal of Analysis Laboratory

基　　金：2024年南阳师范学院大学生实践教学活动创新项目(SPCP2024);2023年国家级线上线下混合式一流课程—《分析化学》项目资助。

摘　　要：采用多层交替电化学沉积法制备了纳米金(Au)和电还原氧化石墨烯(ERGO)修饰的玻碳电极(GCE)。通过对比不同修饰策略,Au和ERGO交替沉积5组所制备的修饰电极(5-Au/ERGO/GCE)对葡萄糖表现出优异的电催化性能。利用扫描电镜(SEM)、能量散射光谱(EDS)和电化学方法对修饰电极进行了表征。在最佳条件下,修饰电极检测葡萄糖的线性范围分别为0.005~1 mmol/L和1.5~7.5 mmol/L,检出限为2.1μmol/L,灵敏度分别为130.27μA/(mmol/L)·cm^(2)和73.53μA/(mmol/L)·cm^(2)。所制备的传感器已成功应用于人血清样品中葡萄糖浓度的测定。Gold(Au) nanoparticles and electro-reduced graphene oxide(ERGO) modified glassy carbon electrodes(GCE) were prepared through multilayer alternating electrochemical deposition.Comparing different modification strategies,the modified electrode of 5-Au/ERGO/GCE exhibited superior electrocatalytic performance for glucose.Characterization of the modified electrode was conducted using scanning electron microscopy(SEM),energy dispersive spectroscopy(EDS),and electrochemical methods.Under optimal conditions,the modified electrode can detect glucose in two linear ranges of 0.005-1 mmol/L and 1.5-7.5 mmol/L,respectively,with a limit of detection of 2.1 μmol/L.The sensor exhibited high sensitivities of 130.27 μA/(mmol/L)·cm~2 and 73.53 μA/(mmol/L)·cm~2 for respective linear ranges.Furthermore,the prepared sensor has been successfully used to analyze glucose concentrations in human serum samples.

关 键 词：多层组装 金 电还原氧化石墨烯 修饰电极 非酶葡萄糖传感 

分 类 号：O657.1[理学—分析化学]