肝脏FATP5表达对MAFLD小鼠LCFA水平的影响  

Effects of hepatic fatty acid transporter 5 expression on long-chain fatty acid levels in mice with metabolic associated fatty liver disease

作　　者：卢毅 吕娇健[1] 赵园 倪霜玲[1] 龙思琴 刘清秀 Lu Yi;Lyu Jiaojian;Zhao Yuan;Ni Shuangling;Long Siqin;Liu Qingxiu(Department of Hepatology/Department of Infectious Diseases,Lishui People's Hospital,Lishui 323000,Zhejiang Province,China)

机构地区：[1]丽水市人民医院肝病科/感染科,丽水323000

出　　处：《中国基层医药》2025年第3期392-396,共5页Chinese Journal of Primary Medicine and Pharmacy

基　　金：浙江省医药卫生科技项目(2022KY1444)。

摘　　要：目的探讨肝脏脂肪酸转运蛋白5基因(FATP5)表达对代谢相关脂肪性肝病(MAFLD)小鼠游离长链脂肪酸(LCFA)水平的影响。方法2022年6—12月进行前瞻性研究,实验性研究野生型(WT)组、脂肪酸转运蛋白5基因表达阴性(FATP5-)组和人脂肪酸转运蛋白5基因表达阳性(hFATP5+)组,每组各10只。各组小鼠高脂食物喂养16周,构建MAFLD小鼠模型。采用HE染色观察肝脏组织变化;测定小鼠肝脏质量和肝脏系数;采用全自动生化分析仪测定小鼠总胆固醇(TC),三酰甘油(TG),天冬氨酸氨基转移酶(AST),丙氨酸氨基转移酶(ALT),尿酸(UA),LCFA水平;采用血糖分析仪测定血糖(Glu)水平。比较三组小鼠肝脏质量和肝脏系数、TC和TG水平、AST和ALT水平、Glu和UA水平及LCFA水平。结果WT组小鼠肝细胞内存在明显炎性细胞浸润情况,有大量脂肪堆积;FATP5-组小鼠肝细胞内炎性细胞浸润较轻,轻微脂肪堆积;hFATP5+组小鼠肝细胞内炎性细胞浸润较重,脂肪堆积较多。FATP5-组肝脏质量[(1.27±0.25)g]、肝脏系数(2.38±0.19)、TC[(1.82±0.26)mmol/L]、TG[(0.93±0.24)mmol/L]、AST[(169.95±37.73)U/L]、ALT[(95.36±21.49)U/L]、Glu[(8.34±1.52)mmol/L]、UA[(74.32±15.52)μmol/L]均低于WT组[(1.61±0.23)g、(2.71±0.20)、(2.31±0.28)mmol/L、(1.34±0.21)mmol/L、(278.31±43.24)U/L、(147.32±28.81)U/L、(10.52±1.24)mmol/L、(96.28±17.43)μmol/L],而LCFA[(3.57±0.48)mg/L]高于WT组[(2.63±0.56)mg/L],差异均有统计学意义(t=3.17、3.78、4.06、4.07、5.97、4.57、3.51、2.98、4.03,均P<0.05)。hFATP5+组肝脏质量[(1.92±0.30)g]、肝脏系数(2.95±0.23)、TC[(2.59±0.24)mmol/L]、TG[(1.76±0.35)mmol/L]、AST[(341.22±48.98)U/L]、ALT[(189.45±17.97)U/L]、Glu[(13.21±1.98)mmol/L]、UA[(117.74±18.38)μmol/L]均高于WT组,而LCFA[(3.57±0.48)mg/L]低于WT组,差异均有统计学意义(t=2.59、2.49、2.40、3.25、3.04、3.92、3.64、2.68、3.19,均P<0.05)。结论肝脏FATP5-可增加MAFLD小鼠血液LCFA水平,抑制小鼠摄�Objective To investigate the effects of hepatic fatty acid transporter 5(FATP5)gene expression on the levels of free long-chain fatty acid(LCFA)in mice with metabolic associated fatty liver disease(MAFLD).Methods From June to December 2022,a prospective study was conducted with three experimental groups:wild-type(WT)group,FATP5 gene expression negative(FATP5-)group,and human FATP5 gene expression positive(hFATP5+)group,with 10 mice in each group.Each group of mice was fed a high-fat diet for 16 weeks to establish a model of MAFLD.Hepatic tissue changes were observed using hematoxylin-eosin staining.The liver mass and liver coefficient of the mice were measured.Total cholesterol(TC),triglycerides(TG),aspartate aminotransferase(AST),alanine aminotransferase(ALT),uric acid(UA),and LCFA levels were determined using an automatic biochemical analyzer.Blood glucose(Glu)levels were measured using a blood glucose analyzer.The liver mass and liver coefficient,TC and TG levels,AST and ALT levels,Glu and UA levels,and LCFA levels were compared among the three groups.Results In the WT group,there was significant inflammatory cell infiltration within the hepatocytes and a large amount of fat accumulation.In the FATP5-group,the inflammatory cell infiltration in the hepatocytes was mild with slight fat accumulation.In the hFATP5+group,the inflammatory cell infiltration in the hepatocytes was severe,with great fat accumulation.The liver mass[(1.27±0.25)g],liver coefficient(2.38±0.19),TC[(1.82±0.26)mmol/L],TG[(0.93±0.24)mmol/L],AST[(169.95±37.73)U/L],ALT[(95.36±21.49)U/L],Glu[(8.34±1.52)mmol/L],and UA[(74.32±15.52)μmol/L]in the FATP5-group were all significantly lower than those in the WT group[(1.61±0.23)g,(2.71±0.20),(2.31±0.28)mmol/L,(1.34±0.21)mmol/L,(278.31±43.24)U/L,(147.32±28.81)U/L,(10.52±1.24)mmol/L,(96.28±17.43)μmol/L],while the LCFA level[(3.57±0.48)mg/L]in the FATP5-group was significantly higher than that in the WT group[(2.63±0.56)mg/L](t=3.17,3.78,4.06,4.07,5.97,4.57,3.51,2.98,4.03,all P<0.05).In

关 键 词：脂肪肝 代谢疾病 脂肪酸转运蛋白质类 基因 脂肪酸类 肝功能试验 血糖 尿酸 

分 类 号：R28[医药卫生—中药学]