低氧条件下丹栀逍遥散对乳腺癌细胞增殖侵袭及缺氧相关因子的影响  

Exploring the Effect of Danzhi Xiaoyao Powder on proliferation and invasion and hypoxia-re⁃lated factors of breast cancer cells under hypoxic conditions

作　　者：李慧[1] 李然[1] LI Hui;LI Ran(Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110032

出　　处：《时珍国医国药》2025年第4期651-657,共7页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(81673876)。

摘　　要：目的研究丹栀逍遥散含药血清在低氧条件下对乳腺癌MDA-MB-231细胞增殖、侵袭及缺氧相关因子表达的影响。方法噻唑蓝比色法(MTT)检测细胞增殖,将MDA-MB-231细胞分为常氧空白组、低氧空白组、丹栀逍遥散组(含药血清10%、15%、20%),培养24,48,72h,观察药物对细胞增殖的抑制作用;通过划痕实验观察各组细胞迁移侵袭情况;蛋白免疫印迹法(WB)检测各组低氧诱导因子-1α(HIF-1α)、单羧酸转运蛋白1/4(MCT1/4)、小窝蛋白-1(CAV-1)及趋化因子受体4(CXCR4)的蛋白表达情况。结果MTT结果表明,低氧条件下,丹栀逍遥散组可显著抑制细胞增殖(P<0.05),其中20%含药血清孵育48h抑制率最佳。划痕实验结果表明,丹栀逍遥散组(20%)细胞迁移率明显下降(P<0.01)。WB实验结果表明,低氧条件下,丹栀逍遥散组(20%)的HIF-1α、MCT1、MCT4、CXCR4的蛋白表达降低(P<0.05、P<0.01)、CAV-1的表达升高(P<0.05)。结论丹栀逍遥散含药血清在低氧条件下能明显抑制MDA-MB-231乳腺癌细胞增殖、迁移侵袭,发挥抗癌作用,可能与其调控缺氧相关乳酸代谢及上皮间质转化(EMT)有关。Objective To investigate the effects of Danzhi Xiaoyao Powder(DZXYP)-containing serum on the proliferation,invasion and expression of hypoxia-related factors in MDA-MB-231 breast cancer cells under hypoxic conditions.Methods Cell proliferation was assessed using the MTT assay.MDA-MB-231 cells were divided into the normoxic control group,the hypoxic control group,and the DZXYP group(with serum oncentrations of 10%,15%,and 20%).The cells were cultured for 24,48,and 72 hours to observe the inhibitory effects of the drug on cell proliferation.A scratch assay was performed to assess the cell migration and invasion in each group.Western blot(WB)was used to detect the protein expression levels of hypoxia-inducible factor-1α(HIF-1α),monocarboxylate transporters 1/4(MCT1/4),caveolin-1(CAV-1),and CXC chemokine receptor 4(CXCR4)in each group.Results The MTT as⁃say results indicated that under hypoxic conditions,cell proliferation was significantly inhibited in the DZXYP group(P<0.05),with the highest inhibition rate observed at 48 hours using 20%serum.The scratch assay results showed a significant decrease in cell migra⁃tion rate in the DZXYP group(20%)(P<0.01).WB analysis revealed that under hypoxic conditions,the protein expression levels of HIF-1α,MCT1,MCT4,and CXCR4 were decreased in the DZXYP group(20%)(P<0.05,P<0.01),while the expression of CAV-1 was increased(P<0.05).Conclusion DZXYP-containing serum can significantly inhibit the proliferation,migration and in⁃vasion of MDA-MB-231 breast cancer cells under hypoxic conditions,thus exerting anti-cancer effects.This may be related to its regulation of hypoxia-associated lactate metabolism and epithelial-mesenchymal transition(EMT).

关 键 词：乳腺癌 丹栀逍遥散 低氧 增殖 侵袭 

分 类 号：R285.5[医药卫生—中药学]