Coupling of alternative splicing and alternative polyadenylation  

作　　者：Xueying Zhang Feiyan Liu Yu Zhou 

机构地区：[1]College of Life Sciences,TaiKang Center for Life and Medical Sciences,Hubei Key Laboratory of Cell Homeostasis,RNA Institute,Wuhan University,Wuhan 430072,China [2]Frontier Science Center for Immunology and Metabolism,Wuhan University,Wuhan 430072,China

出　　处：《Acta Biochimica et Biophysica Sinica》2025年第1期22-32,共11页生物化学与生物物理学报(英文版)

基　　金：supported by the grants from the National Natural Science Foundation of China(No.82341023);the National Key R&D Program of China(No.2023YFC2307802);the Fundamental Research Funds for the Central Universities(No.2042022dx0003)to Y.Z.

摘　　要：RNA splicing and 3′-cleavage and polyadenylation(CPA)are essential processes for the maturation of RNA.There have been extensive independent studies of these regulated processing events,including alternative splicing(AS)and alternative polyadenylation(APA).However,growing evidence suggests potential crosstalk between splicing and 3′-end processing in regulating AS or APA.Here,we first provide a brief overview of the molecular machines involved in splicing and 3′-end processing events,and then review recent studies on the functions and mechanisms of the crosstalk between the two processes.On the one hand,3′-end processing can affect splicing,as 3′-end processing factors and CPA-generated polyA tail promote the splicing of the last intron.Beyond that,3′-end processing factors can also influence the splicing of internal and terminal exons.Those 3′-end processing factors can also interact with different RNA-binding proteins(RBPs)to exert their effects on AS.The length of 3′untranslated region(3′UTR)can affect the splicing of upstream exons.On the other hand,splicing and CPA may compete within introns in generating different products.Furthermore,splicing within the 3′UTR is a significant factor contributing to 3′UTR diversity.Splicing also influences 3′-end processing through the actions of certain splicing factors.Interestingly,some classical RBPs play dual roles in both splicing and 3′-end processing.Finally,we discuss how long-read sequencing technologies aid in understanding the coordination of AS-APA events and envision that these findings may potentially promote the development of new strategies for disease diagnosis and treatment.

关 键 词：alternative polyadenylation alternative splicing CROSSTALK RNA processing RNA regulation 

分 类 号：F23[经济管理—会计学]