HPLC法同时测定蒙药棉团铁线莲中槲皮素和山柰素的含量  

Simultaneous determination of quercetin and kaempferol in Mongolian medicine Clematis hexapetala by HPLC method

作　　者：乌日汗[1] 包同力嘎[2] 额尔敦呼 白明君 乌日汉[1] 苏门 包桂花[1] WURIHAN;BAOTONGLIGA;ERDUNHU;BAI Mingjun;WURIHAN;SU Men;BAO Guihua(Mongolian Medical College,Inner Mongolia Minzu University,Inner Mongolia,Tongliao 028000,China;Department of Clinical Mongolian Medicine,Affiliated Hospital of Inner Mongolia Minzu University,Inner Mongolia,Tongliao 028000,China;Preparation Center of National Mongolian Medicine,International Mongolian Hospital of Inner Mongolia,Inner Mongolia,Hohhot 010020,China)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028000 [2]内蒙古民族大学附属医院临床蒙药剂部,内蒙古通辽028000 [3]内蒙古自治区国际蒙医医院国家蒙药制剂中心,内蒙古呼和浩特010020

出　　处：《中国医药科学》2025年第5期53-56,75,共5页China Medicine And Pharmacy

基　　金：内蒙古自治区自然科学基金项目(2023LHMS08047);内蒙古自治区蒙医药标准化项目(2023-MB030)。

摘　　要：目的通过分析不同产地棉团铁线莲中槲皮素和山柰素的含量,为建立蒙药棉团铁线莲质量控制提供依据。方法采用高效液相色谱法,测定槲皮素和山柰素的含量。结果槲皮素和山柰素分别在0.0389~0.5835μg、0.0746~1.1190μg范围内与峰面积具有很好的线性关系,回收率分别为97.40%和94.30%,相对标准偏差分别为1.36%和1.29%(n=6)。结论该方法分离度好,精密度高,可用于棉团铁线莲中槲皮素和山柰素的含量测定。Objective To provide a basis for establishing quality control of Mongolian medicine Clematis hexapetala by analyzing the content of quercetin and kaempferol in Clematis hexapetala from different origins.Methods High performance liquid chromatography was used to determine th e content of quercetin and kaempferol.Results Quercetin and kaempferol showed good linear relationships with peak area in the ranges of 0.0389-0.5835μg and 0.0746-1.1190μg,respectively.The recovery rates were 97.40%and 94.30%,and the relative standard deviations were 1.36%and 1.29%(n=6),respectively.Conclusion This method has good separation accuracy and high precision,and can be used for the determination of quercetin and kaempferol content in Clematis hexapetala.

关 键 词：蒙药 棉团铁线莲 槲皮素 山柰素 高效液相色谱法 

分 类 号：R29[医药卫生—民族医学]