外周血circRNA在乙肝相关性肝硬化患者中的差异表达与诊断价值研究  

Research on the differential expression and diagnostic value of circRNA in peripheral blood in patients with hepatitis B virus-related cirrhosis

作　　者：叶敏南 何月敬 丁瑜 李国斌 黎四平 YE Minnan;HE Yuejing;DING Yu;LI Guobin;LI Siping(Department of Clinical Laboratory,Dongguan Eighth People’s Hospital,Guangdong,Dongguan 523000,China;Department of Gastroenterology,Dongguan Eighth People’s Hospital,Guangdong,Dongguan 523000,China)

机构地区：[1]东莞市第八人民医院检验科,广东东莞523000 [2]东莞市第八人民医院消化内科,广东东莞523000

出　　处：《中国医药科学》2025年第5期150-154,共5页China Medicine And Pharmacy

基　　金：广东省东莞市社会发展科技(面上)项目(20231800903772)。

摘　　要：目的探讨乙型肝炎病毒(HBV)相关性肝硬化中差异表达的外周血环状RNA(circRNA),并筛选合适的诊断标志物,为乙肝相关性肝硬化患者的早期诊断提供研究基础。方法选取2023年4月至2024年4月东莞市第八人民医院确诊为乙型肝炎患者,且经肝穿刺病理诊断为乙肝相关性肝硬化早期且肝纤维测定值相同的患者10例作为试验组,另选取同期健康体检者10名作为对照组,采用circRNA-seq技术(circRNAseq)检测两组外周血中的circRNA表达谱,使用数据库进行组间差异分析,并筛选合适的差异表达circRNA,作为诊断标志物。基于此,再选择30例乙肝相关性肝硬化患者和30名健康体检者,采用实时荧光定量PCR技术(RT-qPCR)验证两组外周血中相应circRNA的差异表达水平,并建立受试者工作特征曲线(ROC),评估外周血circRNA检测对乙肝相关性肝硬化的诊断价值。结果试验组和对照组外周血中共检测出7419个circRNAs,试验组与对照组相比,其中有349个差异表达circRNA,227个上调、122个下调;hsa_circ_0005632和hsa_circ_0101617作为两个上调circRNA,其在外周血的表达水平显著高于对照组(P<0.05)。对以上两个circRNA进行ROC分析,曲线下面积(AUC)结果显示为0.90、0.94,两者均具有良好的诊断效能,hsa_circ_0101617诊断效能更佳。结论乙肝相关性肝硬化患者的外周血中存在丰富的差异表达circRNA,其中hsa_circ_0005632和hsa_circ_0101617可作为HBV相关性肝硬化的新型诊断标志物。Objective To investigate the differential expression of circular RNA(circRNA)in peripheral blood in hepatitis B virus(HBV)-related cirrhosis,and screen suitable diagnostic markers to provide research basis for early diagnosis of patients with hepatitis B cirrhosis.Methods Patients diagnosed with hepatitis B and admitted to Dongguan Eighth People’s Hospital,who were diagnosed with liver cirrhosis in the early stage by liver puncture and had the same liver fiber measurement value from April 2023 to April 2024 were selected and divided into the experimental group(n=10),and other healthy people in the same period were selected and divided into the control group(n=10).circRNA-seq technology was used to detect the expression profile of circRNA in the peripheral blood of the two groups,the database was used to analyze the differences between the two groups,and the appropriate differential expression of circRNA was screened as a diagnostic marker.Based on this,30 patients with hepatitis B cirrhosis and 30 healthy people were selected.The differential expression level of corresponding circRNA in peripheral blood of the two groups was verified by real-time quantitative polymerase chain reaction(RT-qPCR),and the receiver operating characteristic curve(ROC)was established to evaluate the diagnostic value of circRNA detection in peripheral blood for hepatitis B cirrhosis.Results A total of 7419 circRNAs were detected in the peripheral blood of the experimental group and the control group.Compared with the control group,349 of them in the experimental group expressed circRNA differentially,227 were up-regulated and 122 were down-regulated.hsa_circ_0005632 and hsa_circ_0101617,as two upregulated circRNA,were obviously higher in peripheral blood expression level than those in the control group(P<0.05).ROC analysis of the above two circRNA showed that the area under the curve(AUC)was 0.90 and 0.94.Both of them had good diagnostic efficiency,and hsa_circ_0101617 had superior diagnostic efficiency.Conclusion circRNA is differenti

关 键 词：circRNA 肝硬化 乙型肝炎病毒 生物信息学 诊断标志物 

分 类 号：R512.62[医药卫生—内科学] R575.2[医药卫生—临床医学]