孔氏艾美耳球虫石家庄分离株的致病性和免疫原性研究  

Study on Pathogenicity and Immunogenicity of Eimeria kongi isolated from Shijiangzhuang

作　　者：王鹏[1] 施宇博 方素芳[1] 关琛 崔平[1] WANG Peng;SHI Yubo;FANG Sufang;GUAN Chen;CUI Ping(College of Animal Science and Technology,Hebei North University,Zhangjiakou 075000,China;Zhangjiakou animal husbandry technology promotion station,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院动科院,张家口075000 [2]张家口市畜牧技术推广站,张家口075000

出　　处：《中国动物传染病学报》2025年第1期75-81,共7页Chinese Journal of Animal Infectious Diseases

基　　金：河北省自然科学基金和重点基础研究专项(C2019405052);河北省教育厅资助重点项目(ZD2018032);河北省科技厅重点研发计划项目(19226610D)。

摘　　要：为获得孔氏艾美耳球虫的地理分布及不同地理株的致病性和免疫原性。本研究应用单卵囊分离技术,根据形态学特征获得孔氏艾美耳球虫石家庄株(E.kongi-SJZ),基于ITS-1构建系统发育树,进一步鉴定该虫株。同时,研究了孔氏艾美耳球虫石家庄株的致病性和免疫原性。结果显示,孔氏艾美耳球虫石家庄株(E.kongi-SJZ)与孔氏艾美耳球虫张家口株(E.kongi-ZJK)聚为一个小支,位于兔球虫的单系群内,且位于无外残体的姐妹谱系上,该株球虫属于孔氏艾美耳球虫的一个地理株,具有中等致病性及较强的免疫原性。该结果不仅丰富了兔球虫物种多样性,也为兔球虫病防控提供了依据。To investigate the geographical distribution,pathogenicity and immunogenicity of different Eimeria Kongi strains,the Shijiazhuang strain of E.kongi(E.Kongi-SJZ)was isolated using the single oocyst isolation technique and its phylogenetic tree was constructed based on ITS-1 sequence data in this study.At the same time,the pathogenicity and immunogenicity of E.kongi-SJZ were studied to further characterize this strain.The results showed that E.Kongi-SJZ and E.kongi-ZJK were clustered into a small branch,which located in the monophyletic cluster of rabbit coccidia and in the sister lineage without external residuum.This strain belonged to a geographical strain of E.kongi with moderate pathogenicity and strong immunogenicity.The results not only enriched the species diversity of rabbit coccidia but also provided a basis for the prevention and control of rabbit coccidiosis.

关 键 词：兔 孔氏艾美耳球虫 系统发育分析 致病性 免疫原性 

分 类 号：S852.723[农业科学—基础兽医学]