猪腺病毒3型TaqMan探针荧光定量PCR检测方法建立  

Development of a TaqMan Probe PCR Method for Detection of Porcine Adenovirus Type 3

作　　者：解佳 刘静宜 孙彤[2,3] 郭伟强 俞赵荣 陈鸿军 陈立功[1] XIE Jia;LIU Jingyi;SUN Tong;GUO Weiqiang;YU Zhaorong;CHEN Hongjun;CHEN Ligong(Hebei Agricultural University,Hebei 071000,China;Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;Biosafety Research Center,CAAS,Shanghai 200241,China)

机构地区：[1]河北农业大学,河北071000 [2]中国农业科学院上海兽医研究所,上海200241 [3]中国农业科学院生物安全研究中心,上海200241

出　　处：《中国动物传染病学报》2025年第1期106-111,共6页Chinese Journal of Animal Infectious Diseases

基　　金：上海市2020年度“科技创新行动计划”(20140900300);常州市现代农业技术体系项目。

摘　　要：猪腺病毒3型(PAdV-3)导致猪肠道性疾病。为能够快速特异检测该病毒,本研究根据PAdV-3 hexon基因序列保守区设计特异性引物和探针,成功建立了一种针对PAdV-3的TaqMan探针实时荧光定量PCR(qPCR)检测方法。结果显示:该方法特异性好,可鉴别PAdV-3与其他猪病病毒,最低检测限为10 copies/μL,敏感性高于常规PCR 100倍。该检测方法的建立为PAdV-3的快速诊断提供了重要的分子诊断工具。Porcine adenovirus type 3(PAdV-3)is one of the causative agents of porcine intestinal diseases.Here,we designed specific primers and probes targeting PAdV-3 hexon gene and developed a TaqMan probe fl uorescence quantitative polymerase chain reaction(qPCR)method for detection of PAdV-3.The qPCR method developed here showed good specificity as it distinguished PAdV-3 from other swine viruses.The detection limit was 10 copies/μL,which was 100 times high sensitive than conventional PCR.This TaqMan qPCR method could be used for molecular diagnosis of PAdV-3.

关 键 词：猪腺病毒3型 hexon基因 TAQMAN探针 实时荧光定量PCR 

分 类 号：S852.651[农业科学—基础兽医学]