猪巨细胞病毒TaqMan荧光定量PCR检测方法  

A TaqMan Probe Fluorescence Quantitative PCR Method for Detection of PCMV

作　　者：张岳 孙彤 郭洁真 魏晓锋 熊炜 刘静宜 陈鸿军[2] 陈丹清 ZHANG Yue;SUN Tong;GUO Jiezhen;WEI Xiaofeng;XIONG Wei;LIU Jingyi;CHEN Hongjun;CHEN Danqing(Husbandry and Veterinary center of Wujin District,Changzhou 213161,China;Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;Shanghai Laboratory Animal Research Center,Shanghai 201203,China;Technial Center for Animal,Plant and Food Inspection and Quarantine,Shanghai 200135,China)

机构地区：[1]江苏省常州市武进区畜牧兽医中心,常州213161 [2]中国农业科学院上海兽医研究所,上海200241 [3]上海实验动物研究中心,上海201203 [4]上海海关动植物与食品检验检疫技术中心,上海200135

出　　处：《中国动物传染病学报》2025年第1期119-124,共6页Chinese Journal of Animal Infectious Diseases

基　　金：2021-2022年增补常州市现代农业产业技术体系建设项目;上海市2020年度“科技创新行动计划”实验动物研究领域项目(20140900300)。

摘　　要：猪巨细胞病毒(PCMV)感染可导致猪食欲废绝,母猪流产,产木乃伊胎,仔猪发育不良等临床症状,且分布广泛,对异种器官移植也存在潜在威胁。本研究针对PCMV保守基因DPOL设计引物探针,建立TaqMan实时荧光定量PCR方法。结果显示:检测限可达10拷贝,灵敏度高;变异系数<2%,标准曲线R2=0.998,重复性好,具有高特异性;对上海市及江苏省常州市170份血清样本进行检测,5份样品为可疑结果。本研究建立的方法可用于养殖场猪感染PCMV的筛查,对该病防控具有重要意义。Porcine cytomegalovirus(PCMV)infection in pigs causes clinical sign such,as loss of appetite,miscarriage of sows,production of mummified fetuses,and poor development of piglets.The PCMV infection is widely distributed and also poses a potential threat to xenotransplantation.In this study,a primer probe was designed for the PCMV conservative gene DPOL for development of a TaqMan real-time fluorescence quantitative PCR method.The PCR method showed high sensitivity,good repeatability,and high specificity with the detection limit up to 10 copies,the coefficient of variation was less than 1.41%,and the standard curve R 2 at 0.998.A total of 170 serum samples from Shanghai and Changzhou,Jiangsu were tested and 5 samples were found suspicious.This method could be used for screening PCMV infection on pig farms,and was of great significance for the prevention and control of the disease.

关 键 词：猪巨细胞病毒 DPOL基因 TAQMAN探针 实时荧光定量PCR 

分 类 号：S852.61[农业科学—基础兽医学]