基于重组E^(rns)和E2蛋白的检测猪瘟病毒抗体的间接ELISA的建立  

Development of an Indirect ELISA for Detection of Antibodies against Classical Swine Fever Virus Based on Recombinant E^(rns)and E2 Proteins

作　　者：向蕊 朱利塞 赵小刚 应碧云 王贵平 贾爱卿 XIANG Rui;ZHU Lisai;ZHAO Xiaogang;YING Biyun;WANG Guiping;JIA Aiqing(Guangdong Haida Animal Husbandry and Veterinary Research Institute,Guangzhou 511400,China;Guangdong Key Laboratory of Pig raising and Pig Disease Prevention and Control Technology,Guangzhou 511400,China;Sichuan Hailinger Biopharmaceutical Co.,Ltd.,Chengdu 6111303,China;Guangdong Haida Group Co.,Ltd.,Guangzhou 511400,China)

机构地区：[1]广东海大畜牧兽医研究院,广州511400 [2]广东省养猪与猪病防控技术研究企业重点实验室,广州511400 [3]四川海林格生物制药有限公司,成都6111303 [4]广东海大集团股份有限公司,广州511400

出　　处：《中国动物传染病学报》2025年第1期134-140,共7页Chinese Journal of Animal Infectious Diseases

基　　金：广东省科技计划项目(2020B1212070023)。

摘　　要：本研究利用原核系统表达了猪瘟病毒(CSFV)E^(rns)和E2串联蛋白,通过切胶纯化的方法得到高纯度的目的蛋白作为包被抗原,经过各反应条件的优化初步建立ELISA方法,并对方法的特异性、敏感性和重复性进行检验,与商品化试剂盒以及IFA试验进行平行比较,评估方法的准确性。结果表明建立了一种特异性强、敏感性好、准确率高且适用于临床猪瘟抗体检测的间接ELISA方法。In this study,the E^(rns)and E2 tandem proteins of Classical swine fever virus(CSFV)were expressed in prokaryotic system and the highly purified target proteins were obtained by gel cut purification.The ELISA method was developed by using the purified E^(rns)and E2 tandem proteins as the coating antigens and optimizing the reaction conditions.The specificity,sensitivity and repeatability of the method were tested and compared with commercial kit and IFA test to evaluate the accuracy of the method.The results showed that an indirect ELISA method with strong specificity,good sensitivity,high accuracy was developed in this study and suitable for clinical testing of classical swine fever antibodies.

关 键 词：猪瘟病毒 E2蛋白 E^(rns)蛋白 串联表达 ELISA 

分 类 号：S852.4[农业科学—基础兽医学]