阿拉伯宝螺来源真菌-细菌共生体Aspergillus spelaeus GXIMD 04541/Sphingomonas echinoides GXIMD 04532的次级代谢产物研究  

Secondary metabolites from the fungal-bacterial symbiont Aspergillus spelaeus GXIMD 04541/Sphingomonas echinoides GXIMD 04532 derived from Mauritia arabica

作　　者：杨洁 姚飞华 李晓燕 石婕妤 易湘茜 高程海 YANG Jie;YAO Feihua;LI Xiaoyan;SHI Jieyu;YI Xiangxi;GAO Chenghai(Institute of Marine Drugs,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory of Marine Drugs,Nanning 530200,China;Faculty of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学海洋药物研究院,广西南宁530200 [2]广西海洋药物重点实验室,广西南宁530200 [3]广西中医药大学药学院,广西南宁530200

出　　处：《热带海洋学报》2025年第2期56-63,共8页Journal of Tropical Oceanography

基　　金：国家自然科学基金项目(U20A20101);广西重点研发计划项目(AB24010109);广西海洋药物重点实验室项目(2302603);广西中医药高层次人才传承创新团队项目(2022A007)。

摘　　要：为了研究阿拉伯宝螺(Mauritia arabica)来源的真菌细菌共生体Aspergillus spelaeus GXIMD 04541/Sphingomonas echinoides GXIMD 04532的次级代谢产物,利用硅胶柱层析、凝胶柱层析以及半制备高效液相色谱(high performance liquid chromatography,HPLC)对菌株发酵提取物进行分离纯化,运用现代波谱学方法并结合相关文献数据比对,鉴定化合物结构,通过溴化噻唑蓝四氮唑(methylthiazolyldiphenyl tetrazolium bromide,MTT)法评价化合物细胞毒活性。GXIMD 04541/04532的大米发酵提取物中分离得到5个细胞松弛素类化合物和2个苯内酯类化合物,分别为trichalasins C (1)、aspochalasin E (2)、aspochalasin H (3)、aspochalasin I (4)、aspochalasin K (5)、citreofuran (6)、cyclothiocurvularin B methyl ester (7)。活性测试结果显示,化合物2、4和5表现出PC3细胞毒活性,半数最大抑制浓度(half maximal inhibitory concentration,IC_(50))分别为17.23、15.18和8.71μmol·L^(-1),化合物1—5未表现出22Rv1细胞毒性。In order to investigate the secondary metabolites of the fungal-bacterial symbiont Aspergillus spelaeus GXIMD 04541/Sphingomonas echinoides GXIMD 04532 derived from Mauritia arabica,a series of isolation and purification techniques,including silica gel chromatography,gel column chromatography,and semi-preparative high performance liquid chromatography(HPLC),were employed.The structures of compounds were identified by spectroscopic analysis and literature comparison.Cytotoxic activity evaluation of compounds was conducted using the MTT(Methylthiazolyldiphenyl tetrazolium bromide) assay.Consequently,seven compounds were obtained from rice fermentation extracts of symbiont GXIMD 04541/04532,which were identified as trichalasins C(1),aspochalasin E(2),aspochalasin H(3),aspochalasin I(4),aspochalasin K(5),citreofuran(6),and cyclothiocurvularin B methyl ester(7).Activity assays demonstrated that compounds 2,4,and 5 exhibited cytotoxic activity against PC3 cells,with IC_(50)(half maximal inhibitory concentration) values of 17.23,15.18 and 8.71 μmol·L^(-1),respectively.However,compounds 1-5 showed no cytotoxicity against 22Rv1 cells.

关 键 词：阿拉伯宝螺 真菌–细菌共生体 次级代谢产物 细胞毒活性 

分 类 号：P735.2[天文地球—海洋生物学]