金/鸟氨酸传感器对奥司他韦的检测及与DNA作用  

Detection of oseltamivir phosphate by gold/ornithine sensor and its interaction with DNA

作　　者：孙章华 陈美凤 马心英 吕惠萍 SUN Zhanghua;CHEN Meifeng;MA Xinying;LHuiping(Heze Ecological Environmental Monitoring Center of Shandong Province,Heze 274000,China;College of Chemistry and Chemical Engineering,Heze University,Heze 274015,China)

机构地区：[1]山东省菏泽生态环境监测中心,山东菏泽274000 [2]菏泽学院化学化工学院,山东菏泽274015

出　　处：《传感器与微系统》2025年第4期21-25,30,共6页Transducer and Microsystem Technologies

基　　金：国家自然科学基金资助项目(21875059)。

摘　　要：磷酸奥司他韦(OP)是一种神经氨酸酶抑制剂,临床上用于治疗A和B型流感病毒。实验通过在最佳聚合条件下,在玻碳电极(GCE)上电化学聚合纳米金(AuNPs)/聚L-鸟氨酸(PL-Orn),制备了一种用于测定OP的高灵敏度复合传感器(AuNPs/PL-Orn/GCE)。采用循环伏安法(CV)和扫描电镜(SEM)对该电极进行了表征:PL-Orn在GCE表面形成了一层均匀的膜,AuNPs在PL-Orn均匀地分散,呈针簇状。在最佳测定条件下,该修饰电极可用于OP的测定,线性范围为8.00×10^(-8)~2.00×10^(-5)mol/L,相关系数R为0.9941,检出限为4.00×10^(-8)mol/L。把该电极用于OP胶囊样品的测定,结果满意。利用电化学方法和紫外可见光谱研究了OP与DNA的相互作用,结果表明:OP与DNA发生了增色效应。Oseltamivir phosphate(OP),a neuraminidase inhibitor,is used clinically to treat influenza A and influenza B.A sensor of poly(AuNPs)/poly(L-Ornithine)gold nanoparticles modified glassy carbon electrode,AuNPs/PL-Orn/GCE for determination of OP is fabricated by electropolymerizing AuNPs and PL-Orn on a GCE under optimal polymerization conditions.The electrode is characterized by cyclic voltammetry(CV)and scanning electron microscopy(SEM).The images show that the PL-Orn formed a layer of uniform membrane,and the deposited AuNPs are uniformly dispersed on the PL-Orn/GCE,which is given shape in the form of lusters of needle.The electrochemical experiments show that,under the optimal conditions,the modified electrode can be used for determination of OP.The linear range of 8.00×10^(-8)~2.00×10^(-5)mol/L,with a correlation coefficient R of 0.9941 and a detection limit of 4.00×10^(-8)mol/L is obtained.The practical application of AuNPs/PL-Orn/GCE is demonstrated by the determination of OP in OP capsules samples.This electrochemical method and UV-vis spectrum are employed to investigate the interaction between OP with DNA.The results show that OP and DNA have hyperchromic effect.

关 键 词：鸟氨酸 修饰电极 磷酸奥司他韦 循环伏安法 差分脉冲法 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]