模块化便携相位显微成像系统设计  

Modular and portable phase microscopy system design

作　　者：周扬 廖福元 曹皓月 彭贺 杨树蔚 苏存龙 ZHOU Yang;LIAO Fuyuan;CAO Haoyue;PENG He;YANG Shuwei;SU Cunlong(School of Electronice Information Engineering,Xi’an Technological University,Xi’an 710021,China;School of Intelligent Science and Information Engineering,Xi’an Peihua University,Xi’an 710125,China)

机构地区：[1]西安工业大学电子信息工程学院,西安710021 [2]西安培华学院智能科学与信息工程学院,西安710125

出　　处：《激光杂志》2025年第3期15-21,共7页Laser Journal

基　　金：陕西省教育厅一般专项科学研究计划项目(No.23JK0586)。

摘　　要：传统显微成像技术会对透明细胞活性造成不可逆损伤,且现有光强传输方程缺乏便携性和成本低要求的实用仪器。针对此问题,设计一种基于光强传输方程的便携式显微成像系统。根据光强传输方程的需求自主设计了机械结构并开发配套的交互软件。在保证系统分辨率的前提下,实现了体积缩小70%、高度集成化、快速拆卸组装、成本显著降低等特点。实验结果表明,该系统在10倍物镜下分辨率达到2.19μm,可实时对透明细胞进行相位定量观测,并应用于透明/半透明样本的缺陷检测。该研究为实现低成本、便携性、模块化设计的显微成像技术提供可行性。Conventional microscopy imaging techniques can cause irreversible damage to the activity of transparent cells,while existing light intensity transfer equations lack practical instruments that are both portable and low-cost.To address this challenge,this paper designs a portable microscopic imaging system based on the light intensity transfer equation.According to the requirements of the light intensity transmission equation,the mechanical structure was independently designed and corresponding interactive software was developed.On the premise of ensuring system resolution,it has achieved features such as volume reduction of 70%,high integration,rapid disassembly and assembly,and significant cost reduction.The experimental results demonstrate a resolution of 2.19 microns for the system when using a 10× objective lens.Real time phase quantitative observation of transparent cells can be performed and applied to defect detection of transparent/semi transparent samples.This study provides feasibility for achieving low-cost,portable,and modular design of microscopy imaging technology.

关 键 词：显微成像系统 相位成像技术 光强传输方程 缺陷检测 

分 类 号：O436[机械工程—光学工程]