一种复方消毒液的制备及其抑菌效果  

Preparation of a compound disinfectant and its antibacterial effect

作　　者：郭鑫瑶 程敏 吴辉 王海洁 李小兵 王梅竹 罗罡 王丰 廖万清 康颖倩 GUO Xinyao;CHENG Min;WU Hui;WANG Haijie;LI Xiaobing;WANG Meizhu;LUO Gang;WANG Feng;LIAO Wanqing;KANG Yingqian(Department of Microbiology,Basic Medical College,Guizhou Medical University,Guiyang 550025,Guizhou,China;Guizhou Talent Base for Research on the Relationship between Microbiology and Human Health&Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou,Guiyang 550025,Guizhou,China;Key Laboratory of Environmental Pollution and Disease Control of Ministry of Education,Guizhou Medical University,Guiyang 550025,Guizhou,China;Guizhou Chishui Riverside Maotai-flavor Liquor Research Center,Guiyang 550003,Guizhou,China;Guizhou Provincial Academician Workstation of Microbiology and Health,Guizhou Academy of Tobacco Science,Guiyang,Guizhou,550000,China;Shanghai Key Laboratory of Molecular Medical Mycology,Shanghai 200003,China;Department of Dermatology,Shanghai Changzheng Hospital,Shanghai 200003,China)

机构地区：[1]贵州医科大学基础医学院微生物学教研室,贵州贵阳550025 [2]贵州省微生物与人类健康关系研究人才基地&贵州省普通高校病原生物学特色重点实验室,贵州贵阳550025 [3]贵州医科大学环境污染与疾病监控教育部重点实验室,贵州贵阳550025 [4]贵州省赤水河畔酱酒研究中心,贵州贵阳550003 [5]贵州省微生物与健康院士工作站贵州省烟草科学研究院,贵州贵阳550000 [6]上海市医学真菌分子生物学重点实验室,上海200003 [7]海军军医大学长征医院皮肤科,上海200003

出　　处：《贵州医科大学学报》2025年第3期411-416,共6页Journal of Guizhou Medical University

基　　金：贵州省科技计划项目(黔科合支撑[2020]4Y220);贵阳市科技计划项目筑科合同[2020]-16-24;高等学校学科创新引智计划资助(D20009);国家自然科学基金(32060034);贵州省第六批人才基地项目(RCJD2018-22);贵州省科学技术厅国际科技合作基地(黔科合平台人才[2020]4101)。

摘　　要：目的以中药(连翘、珊瑚姜)和二氧化氯(ClO_(2))为原料制备一种复方消毒液,测试其抑菌效果。方法利用超声研磨仪辅助制备连翘提取液和纳米化珊瑚姜油,采用纸片法观察以ClO_(2)、连翘提取液和纳米化珊瑚姜油为原料的单组分消毒液对金黄色葡萄球菌、大肠杆菌、白念珠菌的抑菌作用;ClO_(2)-连翘提取液-纳米化珊瑚姜油按1∶1∶1、2∶1∶1、1∶2∶1、1∶1∶2等体积复配复方消毒液,利用纸片法比较不同配比消毒液的抑菌效果;扫描电镜观察单组分消毒液和复配消毒液对菌株细胞壁的损伤程度。结果纳米化珊瑚姜油消毒液对金黄色葡萄球菌、大肠杆菌及白念珠菌的抑菌圈明显大于ClO_(2)和连翘提取液,差异有统计学意义(P<0.05);消毒液复配比例为1∶1∶1时对金黄色葡萄球菌和白念珠菌的抑菌效果最好,差异有统计学意义(P<0.05),复配比为2∶1∶1时对大肠杆菌的抑制效果最好,差异有统计学意义(P<0.05);扫描电镜显示1∶1∶1复配后的消毒液对菌株细胞壁的损伤作用强于单组分消毒液。结论ClO_(2)-连翘提取液-纳米化珊瑚姜油按1∶1∶1复配后的复方消毒液具有更好的抑菌效果。Objective To prepare a compound disinfectant using Lianqiao,Shanhujiang,and chlorine dioxide(ClO_(2))as raw materials,and to test its antibacterial effect.Methods Lianqiao extract and nanosized Shanhujiang oil were prepared using an ultrasonic grinder.The antibacterial activities of single-component disinfectants(ClO_(2),Lianqiao extract,and nanosized Shanhujiang oil)against Staphylococcus aureus(S.aureus),Escherichia coli(E.coli),and Candida albicans(C.albicans)were assessed using the Kirby-Bauer disk diffusion method;Compound disinfectants were prepared at volume ratios of ClO_(2):Lianqiao extract:nanosized Shanhujiang oil as 1∶1∶1,2∶1∶1,1∶2∶1,and 1∶1∶2,and their antibacterial effects were compared using the Kirby-Bauer method;Scanning electron microscopy(SEM)was used to observe the damage caused by single-component and compound disinfectants to the bacterial cell walls.Results The zone of inhibition produced by the nanosized Shanhujiang oil disinfectant against S.aureus,E.coli,and C.albicans was significantly larger than those produced by ClO_(2) and Lianqiao extract(P<0.05);When the ratio of compound disinfectant was 1∶1∶1,it exhibited the best antibacterial effect against S.aureus and C.albicans,with statistically significant differences(P<0.05).The 2∶1∶1 ratio was most effective against E.coli(P<0.05);SEM analysis showed that the 1∶1∶1 compound disinfectant caused more severe damage to bacterial cell walls than single-component disinfectants.Conclusion The compound disinfectant prepared with ClO_(2),Lianqiao extract,and nanosized Shanhujiang oil in 1∶1∶1 ratio exhibits superior antibacterial effects compared to single-component disinfectants.

关 键 词：复方消毒液 二氧化氯 连翘 珊瑚姜 抑菌实验 

分 类 号：R378[医药卫生—病原生物学]