云南省迪庆州间断双线绦虫线粒体基因序列多态性分析  

Sequence Polymorphisms of Digramma interrupta Mitochondrial Genes in Diqing Prefecture,Yunnan Province

作　　者：徐翠蓉 谢昕言 闫大龙 杨建岭 马君 贺君君 杨忠文 杨继生 杨建发 邹丰才 XU Cui-rong;XIE Xin-yan;YAN Da-long;YANG Jian-ling;MA Jun;HE Jun-jun;YANG Zhong-wen;YANG Ji-sheng;YANG Jian-fa;ZOU Feng-cai(College of Veterinary Medicine,Yunnan Agricultural University,Kunming,Yunnan 650201,China;Fish Proliferation and Release Station of Xiaozhongdian Reservoir in Diqing Prefecture,Diqing,Yunnan 674400,China;Green Food Development Center of Diqing Tibetan Autonomous Prefecture,Diqing,Yunnan 674400,China)

机构地区：[1]云南农业大学动物医学院,云南昆明650201 [2]迪庆州小中甸水库鱼类增殖放流站,云南迪庆674400 [3]迪庆藏族自治州绿色食品发展中心,云南迪庆674400

出　　处：《动物医学进展》2025年第4期40-45,共6页Progress In Veterinary Medicine

基　　金：兽医寄生虫学专题课程教学案例库项目(云学委[2021]18号);云南省朱兴全专家工作站项目(202005AF150041);云南农业大学兽医公共卫生省创新团队项目(202105AE160014);云南省教育厅科学研究基金项目(2021J0116)。

摘　　要：为分析间断双线绦虫(Digramma interrupta)的线粒体基因(mtDNA)序列的遗传多态性,探讨其作为分子标记的应用潜力,于云南省迪庆州获得的短须裂腹鱼体内分离出21条间断双线绦虫,分别提取基因组DNA,扩增ATP酶亚基6(p atp 6)、烟酰胺腺嘌呤二核苷酸脱氢酶亚基5(p nad 5)、核糖体RNA小亚基(p rrn S)部分基因,并进行序列分析。基于这3个基因,以多房棘球绦虫为外群,采用贝叶斯法构建进化树,分析间断双线绦虫与其他绦虫之间的系统发育关系。结果发现,p atp 6、p nad 5和p rrn S扩增产物的长度分别为543 bp、628 bp和1133 bp,种内差异依次为0~1.1%、0~1.4%和0~0.4%,在进化树上与间断双线绦虫与舌状绦虫亲缘关系最近。结果表明,3个mtDNA序列变异程度从低到高依次为p rrn S<p atp 6<p nad 5,p nad 5是研究间断双线绦虫遗传多样性的理想线粒体分子标记。To analyze the genetic polymorphisms of genes in mitochondrial DNA(mtDNA)in Digramma interrupta,and explore their application potential as molecular markers,in this study,21 D.interrupta were isolated from Schizothorax wangchiachii,which was obtained in Diqing prefecture,Yunnan province.Genomic DNA was extracted,and paritial ATP synthase subunit 6(p atp 6),nicotinamide adenine dinucleotide dehydrogenase subunit 5(p nad 5),small subunit of ribosomal RNA(p rrn S)of D.interrupta were amplified and sequenced.A phylogenetic tree based on nucleotide sequences p atp 6,p nad 5 and p rrn S was constructed using Bayesian method,choosing Echinococcus multilocularis as an outgroup.The results found that the sizes of p atp 6,p nad 5 and p rrn S were approximately 543 bp,628 bp and 1133 bp,respectively.Intraspecific variation in p atp 6,p nad 5 and p rrn S were 0-1.1%,0-1.4%and 0-0.4%,respectively.D.interrupta and L.intestinalis were most related based on the present phylogenetic analysis.The intraspecific variation of sequences among three mt gene of D.interrupta were as follows:p rrn S<p atp 6<p nad 5.The results of present study also indicated that p nad 5 is an ideal mt molecular marker in studying the genetic diversity of species in genus Digramma.

关 键 词：间断双线绦虫 线粒体DNA 序列分析 进化分析 

分 类 号：S852.734[农业科学—基础兽医学] S865.33[农业科学—兽医学]