益精通络方抑制离焦型高度近视豚鼠近视进展的实验研究  

Experimental Study on the Inhibition of Myopia Progression in Diffusion-induced High Myopia Guinea Pigs by Yijing Tongluo Formula

作　　者：曹丹凤 尹连荣[1] 康婷婷 刘沙沙 张月强 王瑶 于滢 马若楠 CAO Danfeng;YIN Lianrong;KANG Tingting;LIU Shasha;ZHANG Yueqiang;WANG Yao;YU Ying;MA Ruonan(Eye Hospital,China Academy of Chinese Medical Sciences,Beijing 100040,China)

机构地区：[1]中国中医科学院眼科医院,北京100070

出　　处：《中国中医眼科杂志》2025年第4期301-305,共5页China Journal of Chinese Ophthalmology

基　　金：首都卫生发展科研专项重点攻关项目(2022-1-4182);中国中医科学院眼科医院高水平中医医院建设项目(GSP3-12、GSP2-06);中国中医科学院眼科医院院级科研启动基金项目(101110302)。

摘　　要：目的探讨益精通络方(YJTL)对离焦型高度近视(DIHM)豚鼠近视进展的抑制作用。方法将30只三色豚鼠随机分为对照组(CG)、模型组(MG)、益精通络方组(YJTL),每组10只。以右眼作为实验眼,MG、YJTL组豚鼠佩戴诱导头套建立DIHM模型,CG组豚鼠予平光镜镜片头套。YJTL组豚鼠灌胃12.7 g/kg剂量的益精通络方汤剂,CG组、MG组豚鼠灌胃等体积0.9%氯化钠注射液,每日1次,连续干预5周。在干预前、干预3周后及干预5周后分别测量3组豚鼠右眼等效球镜(SE)度数及眼轴长度(AL)。干预5周后采用苏木素-伊红(HE)染色法观察豚鼠巩膜及脉络膜形态。结果(1)SE度数:CG组豚鼠干预后的SE度数均较干预前降低(t_(3周)=13.835、t_(5周)=15.850,均P=0.000),YJTL组、MG组豚鼠干预后的SE度数均较干预前升高,差异均有统计学意义(YJTL:t_(3周)=42.551、t_(5周)=42.236,均P=0.000;MG:t_(3周)=42.230、t_(5周)=54.845,均P=0.000)。干预3周及5周后,MG组豚鼠SE度数高于CG组(t_(3周)=51.492、t_(5周)=108.416,均P=0.000),YJTL组豚鼠SE度数低于MG组(t_(3周)=12.718、t_(5周)=12.425,均P=0.000),差异均有统计学意义。(2)AL:3组豚鼠干预3周后及5周后的AL较干预前增长,差异均有统计学意义(CG:t_(3周)=13.214、t_(5周)=20.138,均P=0.000;MG:t_(3周)=22.748、t_(5周)=31.644,均P=0.000;YJTL:t_(3周)=20.036、t_(5周)=27.385,均P=0.000)。干预3周及5周后,MG组豚鼠AL长于CG组(t_(3周)=16.837、t_(5周)=29.999,均P=0.000),YJTL组豚鼠AL短于MG组(t_(3周)=2.266,P=0.036;t_(5周)=5.784,P=0.000),差异均有统计学意义。(3)巩膜及脉络膜形态:MG组豚鼠脉络膜和巩膜厚度低于CG组(t_(脉络膜)=19.434、t_(巩膜)=271.060,均P=0.000),YJTL组豚鼠脉络膜和巩膜厚度高于MG组(t_(脉络膜)=11.299、t_(巩膜)=10.926,均P=0.000),差异均有统计学意义。结论益精通络方可抑制DIHM豚鼠SE度数增长、AL延长、脉络膜及巩膜变薄,控制近视进展。OBJECTIVE To investigate the inhibitory effect of Yijing Tongluo Formula(YJTL)on the progression of diffusion-induced high myopia(DIHM)in guinea pigs.METHODS A total of 30 three-color guinea pigs were randomly divided into three groups:control group(CG),model group(MG),and Yijing Tongluo Formula group(YJTL),with 10 animals in each group.The right eye was selected as the experimental eye.The DIHM model was induced in the MG and YJTL groups by wearing inducing headsets,while the CG group wore plano lenses.The guinea pigs in the YJTL group were gavaged with 12.7 g/kg of Yijing Tongluo Formula decoction,while the guinea pigs in the CG and MG groups received the same volume of 0.9%saline solution.The treatment was administered once daily for five weeks.Spherical equivalent(SE)and axial length(AL)of the right eye were measured before the intervention,after three weeks,and after five weeks of intervention.After five weeks of intervention,the morphological changes in the sclera and choroid were observed using hematoxylin and eosin(HE)staining.RESULTS(1)SE:In the CG group,SE significantly decreased after intervention(t_(3 weeks)=13.835,t_(5 weeks)=15.850,both P=0.000).In the YJTL and MG groups,SE significantly increased after intervention(YJTL:t_(3 weeks)=42.551,t_(5 weeks)=42.236,both P=0.000;MG:t_(3 weeks)=42.230,t_(5 weeks)=54.845,both P=0.000),with statistically significant differences.After three and five weeks,the SE in the MG group was higher than that in the CG group(t_(3 weeks)=51.492,t_(5 weeks)=108.416,both P=0.000),and the SE in the YJTL group was lower than that in the MG group(t_(3 weeks)=12.718,t_(5 weeks)=12.425,both P=0.000),with significant differences.(2)AL:After three and five weeks of intervention,the AL of all three groups increased significantly(CG:t_(3 weeks)=13.214,t_(5 weeks)=20.138,both P=0.000;MG:t_(3 weeks)=22.748,t_(5 weeks)=31.644,both P=0.000;YJTL:t_(3 weeks)=20.036,t_(5 weeks)=27.385,both P=0.000).After three and five weeks,the AL in the MG group was longer than in the CG group(t_(3 weeks)

关 键 词：益精通络方 高度近视 脉络膜 巩膜 三色豚鼠 

分 类 号：R285.5[医药卫生—中药学] R774.61[医药卫生—中医学]