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作 者:霍天祺 罗崇一 杨瑞菊 袁彪 徐颖瑞 杨卫丽 HUO Tianqi;LUO Chongyi;YANG Ruiju;YUAN Biao;XU Yingrui;YANG Weili(Hainan Medical University,Haikou,Hainan 571199,China)
机构地区:[1]海南医科大学,海南海口571199
出 处:《热带农业科学》2025年第2期49-54,共6页Chinese Journal of Tropical Agriculture
基 金:海南省自然科学基金资助项目(No.820RC624);研究生创新创业项目(No.HYYB2023A17);大学生创新创业项目(No.S202311810007)。
摘 要:植株根际土壤微生态群落与植物的生长发育及代谢密切相关,真菌作为微生物区系重要的组成部分,对植株的生长发育也至关重要。本研究提取胆木植株根际土壤真菌菌群总DNA,PCR后使用高通量测序平台对其进行ITS序列测序。根据有效数据统计、ASV分布统计及Alpha分析进行样品验证;根据样品真菌菌群结构分析可得知,胆木根际土壤真菌群落主要聚集在子囊菌门的漆斑霉属,其次在担子菌门、球囊菌门等菌门也丰度较高。微生物对植物的影响一直是研究热点,胆木根际土壤真菌微生态群落结构分析,可为真菌类微生物与胆木植株间的相关性研究提供一定参考。The rhizosphere soil microecological community is closely related to the growth and metabolism of plants.Fungi,as a vital part of the microbial community,are also crucial to the growth and development of plants.In this study,the total DNA of the fungal community in the rhizosphere soil of the Nauclea officinalis was extracted,and the ITS sequence was sequenced via a high-throughput sequencing platform after PCR.According to the effective data statistics,including ASV distribution statistics and alpha analysis for sample verification,and according to the analysis of the fungal community structure of the sample,it can be known that the fungal community in the rhizosphere soil of N.officinalis was mainly concentrated in the Myrothecium of Ascomycota,followed by Basidiomycota,Glomeromycota and other phyla.The effects of microorganisms on plants have always been a research hotspot.The analysis of its fungal microecological community structure in the rhizosphere soil of N.officinalis can provide some reference for the correlation between fungal microorganisms and N.officinalis plants.
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